胃异位胰腺1例分析并文献复习

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1、羊膜衍生膜负载干细胞通过PI3K/MAPK修复及优化软骨损伤的评估［摘要］H的探？人羊膜衍生膜(humanacellularamnioticmembrane,HAAM)负载干细胞通过PI3K/MAPK修复及优化软骨损伤的作用。方法新西兰白兔分离出骨髓间充质干细胞(bonemesenchymalstemcells,BMSCs)并与软骨细胞按4:1接种于HAAM上，接种后7d和14d观察软骨细胞的修复及优化情况。细胞计数CCK-8法检测软骨细胞的存活率；TUNEL法检测软骨细胞凋亡发生；WesternBlot法检测软骨细胞中I型胶原、II型胶原，磷酸化蛋白p-PKC、p-p38

2、、p-JNK和p-ERK蛋白表达水平。结果与对照组相比，实验组细胞存活率显著升高，细胞凋亡显著降低，差异具有统计学意义(P<0.05);与对照组相比，实验组中I型胶原、II型胶原含景显著增加；PI3K/MAPK信号通路磷酸化蛋白含量变化明显，差异具有统计学意义(P〈0.05)。结论羊膜衍生膜负载骨髓间充质干细胞可以修复和优化软骨的损伤，且PI3K/MAPK信号通路在此过程中有重要作用。本文采集自网络，本站发布的论文均是优质论文，供学习和研究使用，文中立场与本网站无关，版权和著作权归原作者所有，如有不愿意被转载的情况，请通知我们删除匕转载的信息，如果需要分享，请保留本段说明。

3、［关键词］HAAM；BMSCs；软骨细胞；PI3K/WK［中图分类号］R363［文献标识码］A［文章编号］1673-9701(2017)18-0031-04EvaluationofacellularamnioticmembraneloadedstemcellsinrepairingandoptimizingcartilageinjurybyPI3K/MAPKpathwayZHANGZhijinlZENGLinrulZHUFangbinglGUOLin2LIQianxiao31.DepartmentofOrthopaedics，XiaoshanDistrictTraditio

4、nalChineseMedicineHospitalofHangzhouCity，Hangzhou311201，China；2.DepartmentofJointSurgery，ZhongshanHospitalAffiliatedtoDalianUniversity,Dalian116000，China；3.DepartmentofCardiology,HangzhouRedCrossHospital,Hangzhou310000，China［Abstract］ObjectiveToinvestigatetheeffectofhumanacellularamnioticm

5、embrane(HAAM)loadedstemcellsinrepairingandoptimizingcartilageinjurybyPI3K/MAPKpathway.MethodsBonemesenchymalstemcells(BMSCs)wereisolatedfromNewZealandwhiterabbitsandwereplatedon11AAMcombinedwithchondrocytesat4：1.Therepairandoptimizationofchondrocyteswereobservedat7and14daysafterinoculation

6、.ThesurvivalrateofchondrocyteswasdetectedbycellcountCCK-8method.TheapoptosisofchondrocyteswasdetectedbyTUNELmethod.WesternBlotwasusedtodetecttheexpressionoftypeIcollagen，typeIIcollagen，expressionlevelsofphosphorylatedproteinp-PKC,p-p38,p-JNKandp-ERK.ResultsComparedwiththatinthecontrolgroup

7、,thesurvivalrateoftheexperimentalgroupwassignificantlyhigher,andcellapoptosisoftheexperimentalgroupwassignificantlyreduced,andthedifferencewasstatisticallysignificant（P〈0.05）.Comparedwiththoseinthecontrolgroup,thetypeIcollagenandtypeIIcollagencontentintheexper