先张法预应力溷凝土简支空心板设计

摘要:根据细菌

5、的16SrDNA-23SrDNA的高度保守区设计引物，扩增了嗜肺巴斯德杆菌的16S-23SrDNA间区，经测序获得521bp和683bp两条产物。用BLAST和DNAster软件对16S-23SrDNA间区序列进行比较分析，设计出对嗜肺巴斯德杆菌特异的PCR引物，进行了特异性、灵敏性检测分析。为建立检测嗜肺巴斯德杆菌的PCR方法奠定了良好的基础。
关健词:嗜肺巴斯德杆菌；16S-23SrDNA间区；特异性；灵敏性

EstablishmentandapplicationofPasteurellapne

6、umotropicacoli16SrDNA-PCRdetectionmethods
Abstract:Inthisstudy,PCRprimerDesignedfrom16SrDNA-23SrDNAconservedIntergenicSpacerRegionandamplifiedthisregion,andobtainedPCRproductsof521bpand683bp.Theseproductssequenced.UsingBLASTandDNAstarsoftwaretoconductcomparison

7、analysisto16S-23SrDNAPCRproductsspecifictoPasteurelapneumotropica,thenPCRprimerswasconductedtestinganalysisofspecificityandsensitivity.ThepurposetomakeagoodfoundationtoestablishPCR-basedmethodof detectiontoPasteurelapneumotropica.
Keywords:Pasteurelapneumo

8、tropica;16S-23SrDNASequence;Specificity;Sensitivity

目   录  5200字
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