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1、第四军医大学硕士学位论文to0.25mLplasma.Thesampleswereextractedtwicewith2.0mLacetidin.Theorganicphasewastransferredtoacleantubeandevaporatedtodrynessat50℃underagentlestreamofnitrogenintheventilationcabinet.Theresiduewasdissolvedin50μLofmethanoland10μLwastakenforLC/MS/MSanalysis.(2)0.2mLglucuronidases
2、olution(2000U/mL)wasaddedto0.25mLplasmaandincubatedat37℃for24hinwaterbathshake.Thesamplewastreateddetectedaccordingtostep(1).3.Themodelofintestinaltractperfusioninsituwassetup.Theintestinaparvaof10cminlengthfromthepoint5cmbelowtheduodenumwasperfusedbyintubation,andthentheperfusatesamples
3、werecollectedatdifferentpoints(10,20,30,40,50,60min)viaportalvein.TheconcentrationofresveratrolandpolydatinweredetectedbythemethodofLC/MS/MS.Atthesametime,thesamplewastreatedwithglucuronidasetodetecttheconcentrationofglucuronidatedresveratrolandpolydatin.4.Themodelofliverperfusioninsituw
4、assetup.Thedrugwaspouredintoportalvein,andthentheperfusatesampleswerecollectedatdifferentpoints(10,20,30,40,50,60min)viainferiorvenacave.TheconcentrationofresveratrolandpolydatinweredetectedbythemethodofLC/MS/MS.Atthesametime,thesamplewastreatedwithglucuronidasetodetecttheconcentrationof
5、glucuronidatedresveratrolandpolydatin.RESULTS:1.TaketheratioofpeakareaofpolydatinandresveratroltopeakareaofinternalstandardgenisteinseparatelyforY-axis,andtheconcentrationofpolydatinandresveratrolforX-axistobuilttheworkingcurve.Thetypicalregressionequationtoassaytheconcentrationofthepoly
6、datinintheratplasmawasY=0.0543X-0.032，γ=0.9999，n=6.Thelinearrangewas0.4-200ng/mLandthefixedlowerquantitywas0.4ng/mL.Thetypicalregressionequationtoassay6第四军医大学硕士学位论文theconcentrationoftheresveratrolintheratplasmawasY=0.0366X+0.1138，γ=0.9999，n=6.Thelinearrangewas0.4-200ng/mLandthefixedlower
7、quantitywas0.4ng/mL.2.PolydatinwasquicklyabsorbedandbiotransformedinSDrats,andproducedresveratrol,glucuronidatedresveratrolandglucuronidatedpolydatin,etc.AftertheSDratsweregivenatthedoseof50,100and300mg/kgbygavage,theareaundercurve(AUC)ofpolydatinwere53574,85970,228245ng•
