蠲痛饮对子宫内膜异位症大鼠VEGF的影响

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1、目录中文摘要···············································1英文摘要···············································3引言················································6正文················································71.实验材料和方法······································71.1材料··············

2、···································71.2动物饲养条件·········································81.3试验方法·············································81.4指标测定············································101.5统计学处理··········································112.实验结果························

3、····················112.1造膜后异位子宫内膜生长情况··························112.2给药后异位内膜生长情况·······························112.3大鼠血清VEGF水平····································123.讨论·············································133.1EMS概述············································133.2EMS

4、治疗············································163.3关于子宫内膜异位症动物模型的建立·············183.4丹那唑与子宫内膜异位症··························203.5血管内皮生长因子·································213.6蠲痛饮对大鼠子宫内膜异位症的影响···········244.蠲痛饮方解析·······································255.蠲痛饮药物及现代药理研究··········

5、··············266.思考与展望·········································29结论···············································30附图···············································31参考文献··············································32综述···············································37致谢··

6、·············································46攻读学位期间发表的学术论文目录····················47作者声明··············································48个人简历··············································49中文摘要目的：本项目通过深入、系统研究蠲痛饮对血管内皮生长因子(VEGF)的干预作用，从细胞分子水平上研究名老中医治疗妇科痛症、不孕症的学术理论，研究蠲痛饮对子宫内膜异位症（

7、endometriosis，EMS）的作用机理，旨在为中医药治疗EMS的辩病论治及机制探索提供可资借鉴的研究思路，为临床有效治疗EMS提供一个良好的切入点，为推广应用打下坚实的基础。方法：将健康清洁级雌性SD大鼠72只，体重220-250g，随机抽取12只作为空白组，其余大鼠均进行造模，建模方法参照Jones[1]等建模方法，4周末经病理组织学检查证实异位内膜已成活。再将造模成功大鼠随机分为蠲痛饮高、中、低剂量组、丹那唑组、模型组。给予蠲痛饮治疗并与丹那唑为对照，给药12周以后，禁食12小时称重，经股静脉采血，离心后吸取上清液，-20℃低温冰箱

8、中保存备用。运用酶联免疫吸附试验（EliSA）中的双抗体夹心法测定血清VEGF水平，从药物调控VEGF表达的影响进一步研究蠲痛饮对EMS的作用机制。结