血管新生与VEGF表达的影响

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1、华中科技大学硕士学位论文AbstractObjective:ToexploretheeffectsandpossiblemechanismofrecombinanthumanNeuregulin-1βoncardiacfunctionofacutemyocardialinfarction(AMI)rats,theangiogenesisandtheexpressionofvascularendothelialgrowthfactor(VEGF)inischemicmyocardium.Methods:AMImodelwasestablishedwithSprague-Dawleyr

2、atsbyligationoftheleftanteriordescendingcoronaryartery．Theratswhichsurvived24hoursaftersurgerywererandomlyseparatedinto2groups:rhNrg-1βtreatmentgroupwithcaudal veininjectionofrhNrg-1βatadoseofl0µg·kg-1·d–1for5consecutivedaysandthemyocardialinfarction(MI)controlgroup.Anothershamoperationgroupun

3、derwentthoracicincisionwithoutligationofleftanteriordescendingcoronaryartery.MIcontrolgroupandshamoperationgroupweresimultaneouslyadministeredwith0.1mol/LPBS.Afterroutinefeedandwaterfor28days,cardiacfunctionofdifferentgroupswereevaluatedbyechocardiography.Afterthat,theratsweresoonexecutedtodet

4、ecttheweightandmassindexofleftventricular.DensitiesofnewcollateralmicrovesselweremeasuredbyⅧfactorimmunohistochemicalanalysis.WesternblottingwasadoptedtoobserveVEGFexpressionintheischemicmyocardiumofAMIrats.Results:ComparedwiththeMIcontrolgroup,cardiacfunctionoftherhNrg-1βtreatmentgroupobvious

5、lyimproved;Comparedwiththeshamoperationgroup,alargenumberofnewbloodvesselsintheproliferativestagecanbefoundintheinfarctionborderzoneofbothMIgroupandrhNrg-1βtreatmentgroup,andcapillarydensityintherhNrg-1βtreatmentgroupwassignificantlygreaterthanthatintheMIcontrolgroup;Besides,theexpressionofVEG

6、FproteinsignificantlyincreasedintheinfarctionborderzoneofMIgroupandrhNrg-1βtreatmentgroup,andexpressionlevelintheMIgroupwasmuchlessthanthatintherhNrg-1βtreatmentgroup.Conclusions:rhNrg-1βincreaseexpressionofVEGFproteininischemicmyocardiumofAMIratswhichcanpromoteangiogenesisandcollateralcircula

7、tiontoprotectischemicmyocardiumandimprovecardiacfunction.Keywords:rhNrg-1β；acutemyocardialinfarction；angiogenesis；VEGF3华中科技大学硕士学位论文英文缩略语简表Nrg-1βNeuregulin-1β神经调节蛋白-1β rhNrg-1βrecombinanthumanNeuregulin-1β重组人神经调节蛋白-1βAMIAcutemyocardialin