资源描述：
《大亚湾海域微表层和次表层浮游植物群落结构的比较-研究》由会员上传分享，免费在线阅读，更多相关内容在应用文档-天天文库。
1、暨南大学硕士研究生学位论文DNA指纹图谱中，能得到更为全面、准确的蓝细菌分布信息。因此，PCR-DGGE技术可作为传统显微观察分析方法的有效补充，用于浮游植物群落结构分析。关键词：微表层；次表层；浮游植物；PCR-DGGE；DNA指纹；大亚湾II暨南大学硕士研究生学位论文AbstractTheseasurfacemicrolayer(SML)isanimportantinterfacebetweentheatmosphereandthesea.Thisinterfacehasuniquephysicochemicalandbiologicalproperties.Anann
2、ualsurveywasconductedonthephytoplanktoncommunityandenvironmentalfactorsinDapengaoareaofDayaBaybetweenApril2013andMarch2014.Thepurposeofthisstudyistobetterunderstandphytoplanktoncomposition,quantitativedistributionandannualvariationofdominantspecies,andtoknowthecharacteristicsofphytoplankto
3、ncommunitystructureintheSMLandsubsurfacewater(SSW).Meanwhile,phytoplanktonDNAfingerprintswereanalyzedbyPCR-denaturinggradientgelelectrophoresis(PCR-DGGE)technique.TheresultsfromthisstudyenrichedtheinformationofphytoplanktonstructureintheSMLofDayaBay,andprovidedascientificbasisforenvironmen
4、talprotectioninthisseaarea.Atotalof142taxawereidentifiedduringthesurvey,including132speciesfromtheSMLand128fromtheSSW.However,theShannon-Weaverspeciesdiversityindexandevennesswaslow,indicatingthedeteriorationofenvironmentsinDayaBayintherecentyears.Diatomwasthepredominantgroupofphytoplankto
5、n,followedbydinoflagellate,andfewoccurrenceofspeciesinothergroupssuchascyanobateria,chrysophytes，cryptophytesandrhaphidophytes.ThecommonpredominantspeciesincludedChaetoceros,Skeletonemacostatum,Pseudo-nitzschiaandLeptocylindrusdanicus,whichwerepresentedthroughoutthewholeyear.Meanwhile,Rhiz
6、osoleniafragilissima,Cerataulinapelagica,Guinardiastriatadominatedinsomeparticularmonths.Thephytoplanktonabundancewashighaswell,withtheannualaveragecellsdensityof662.07×10cells/Land1.48×10cells/LintheSMLandSSW,respectively.Thecelldensitypeakedinsummer,followedbywinter,andwaslowerinspringan
7、dautumn.AhighpeakofS.costatumoccurredinFebruary,whichresultedinwinterpeakofphytoplankton.ThephytoplanktonstructureintheSMLwassimilartothatintheSSW,andwascharacterizedbythedominanceofdiatoms,highspeciesrichnessandlowspeciesdiversity.Thetotalphytoplankton,diatom
