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1、暨南大学硕士研究生学位论文AbstractTheseasurfacemicrolayer(SML)isanimportantinterfacebetweentheatmosphereandthesea.Thisinterfacehasuniquephysicochemicalandbiologicalproperties.AnannualsurveywasconductedonthephytoplanktoncommunityandenvironmentalfactorsinDapengaoareaofD
2、ayaBaybetweenApril2013andMarch2014.Thepurposeofthisstudyistobetterunderstandphytoplanktoncomposition,quantitativedistributionandannualvariationofdominantspecies,andtoknowthecharacteristicsofphytoplanktoncommunitystructureintheSMLandsubsurfacewater(SSW).Me
3、anwhile,phytoplanktonDNAfingerprintswereanalyzedbyPCR-denaturinggradientgelelectrophoresis(PCR-DGGE)technique.TheresultsfromthisstudyenrichedtheinformationofphytoplanktonstructureintheSMLofDayaBay,andprovidedascientificbasisforenvironmentalprotectioninthi
4、sseaarea.Atotalof142taxawereidentifiedduringthesurvey,including132speciesfromtheSMLand128fromtheSSW.However,theShannon-Weaverspeciesdiversityindexandevennesswaslow,indicatingthedeteriorationofenvironmentsinDayaBayintherecentyears.Diatomwasthepredominantgr
5、oupofphytoplankton,followedbydinoflagellate,andfewoccurrenceofspeciesinothergroupssuchascyanobateria,chrysophytes，cryptophytesandrhaphidophytes.ThecommonpredominantspeciesincludedChaetoceros,Skeletonemacostatum,Pseudo-nitzschiaandLeptocylindrusdanicus,whi
6、chwerepresentedthroughoutthewholeyear.Meanwhile,Rhizosoleniafragilissima,Cerataulinapelagica,Guinardiastriatadominatedinsomeparticularmonths.Thephytoplanktonabundancewashighaswell,withtheannualaveragecellsdensityof662.07×10cells/Land1.48×10cells/LintheSML
7、andSSW,respectively.Thecelldensitypeakedinsummer,followedbywinter,andwaslowerinspringandautumn.AhighpeakofS.costatumoccurredinFebruary,whichresultedinwinterpeakofphytoplankton.ThephytoplanktonstructureintheSMLwassimilartothatintheSSW,andwascharacterizedby
8、thedominanceofdiatoms,highspeciesrichnessandlowspeciesdiversity.Thetotalphytoplankton,diatomsanddinoflagellateswereenrichedintheSML,particularlyfortheIII暨南大学硕士研究生学位论文dominantdiatoms.Theabundancesofeukaryoticphytopla
