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1、山西医科大学硕士学位论文1.Amongthe6kindsofhousekeepinggenes,wasexcludedfromthestatisticswhichwasundetectedinmultiplesamples.B2M,HMBS,HPRT1,OAZ1,ACTBhadgoodstability,thedifferencetestbetweenthetwogroupsshowedallofPvaluesweremorethan0.05.2.88stressrelatedgenesexpressionwere
2、notsamewhencomparedbetweentwogroups.Thereare25genesdifferentialexpressionwithalagermultiple(absolutevalue>2),theywereHRH1、JUN、CCL2、HSPB7、HDAC5、HDAC6、CXCL2、HSPB2、EP300、CCR4、CXCL10、CCL7、CCL5、STAC、HDAC4、MAP2K7、CIDEB、IL9、IL13、IL5、IL1A、CD40LG、HSPB3、SCARA5、FOS.There
3、are13genesexpressionhadstatisticallysignificantdifference,theywereCCL2、CECPB、IL1A、IL13、CD49LG、MAPK1、IL1B、LIG1、CCL7、TAF1、DGKK、CXCL1、CCR2.3.Put13geneswhichdifferentialexpressionwerestatisticallysignificant(P<0.1)intodatabasefortheGOtermenrichmentanalysis,itshowe
4、dthatthemainfunctionofthe13genesembodiedinfouraspects:thefirstOneistheregulationoftheactivityofcytokines,immuneresponseanddamageresponse;theSecondonewasapositiveregulatorofthesynthesisofthesynthesisofmacromoleculesandmacromolecularpositivelyregulatesmetabolism
5、process;Thethirdonewastoregulatecellproliferation;TheFourthonewasregulationofextracellularspace.Conclusion：1.Inthisexperiment,theselectedreferencegenestabilitywasgood,whichwasconsistentwiththeourpreviousexperimentalresults.2.88stressrelatedgenesexpressionweren
6、otsamewhencomparedbetweentwogroups.Thereare25genesdifferentialexpressionwithalagermultiple(absolutevalue>2),thereare13genesexpressionhadstatisticallysignificantdifference(P＜0.1).Theseshowedthattwokindsofdeathprocessinatypicaldrowningandtypicaldrowninghadthedif
7、ferentstressreactions.3.TheexpressionofCCLfamilygenesindeadratslungtissueshadsignificantdifferencesbetweentypicaldrowninggroupandatypicaldrowninggroup,especiallytheVI山西医科大学硕士学位论文Ccl2.Thispromptedustointhenextexperiment,specialattentionshouldbepaidtootherstress
8、generesearchwithinthestressgenelibraryandCCLfamilygenes.Weshouldexpandtheanimalresearchsample,andfinalresearchtheapplicationsofstressrelatedgenesinhumandrownedcorpse.KeyWordsatypic
