基于碳纳米材料检测肿瘤标志物的-研究

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1、基于碳纳米材料检测肿瘤标志物的研究关键词：流动注射；化学发光：荧光检测：肿瘤细胞：谷胱甘肽：碳纳米管：氧化石墨烯。II青岛科技大学研究生学位论文THEDETECTIONOFCANCERMARKERSBASED0NCARBONNANOMATERlALSABSTRACTInthechapterone，firstofall，theprincipleofthechemicalluminescenceandthefluorescencebasicwasintroducedbriefly．Thenthedetectionprincipleandresearchprogressofthebiolog

2、icalsensors(includingchemicalluminescencesensor,thefluorescentsensor)werereviewedindetail，andtheworkforbiologicalsensorswithcarbonnanometermaterialdetectionwassummarized．Inthechaptertwo，Thecarbonnanotubes(CNTs—COOH)andoxidationgraphitesurfaces(GO)ofnanomaterialsweresynthesized，thephaseandmorphol

3、ogyofproductswerecharacterizedbyHighresolutiontransmissionelectronmicroscopyandFouriertransforminfraredspectroscopy．ThemorphologyofOxidationgraphitesurfaces(GO)wereidentifiedasflakestructurebyTEMphotographsandthesmallestpiecessizewasfor50nm．Carbonnanotubes(CNTs—COOH)wastubularnanoscalematerials，

4、nofixedlengthandthediametersizedistributionintherangeof5～10nm．TheFT-IRresultshewthattheCNTs—COOHfunctionalgrouppipecontainsalotofCOOHgroups．Inthechapterthree，TheRNAhadbeenquantitativeanalysisbythedetectionmethodofflowinjectionchemiluminescencebasedontheoxidationofgraphiteenzymecirculationamplifi

5、cationsystem．TheoptimumconditionsofRNAanddetectionwere1．0×10～mol·L～PBS(pH=8．O)forthebuffersolution，8．0×10—3m01．L～fortheH70，solutionconcentration．4．0×10。4m01．L一1fortheLuminolsolutionconcentration．1．0×10～m01．L～fortheHeminsolutionconcentration．reactionparameterssetasfollows，400VfortheNegativepressu

6、re，gainwas1，the40rad／minformainpumprotationspeed，10rad／minfornegativepumprotationspeed，thebestincubationtimeofsystemwas75min．Inthiscondition，thetargetRNAweretested，thelinearrangewas5．0×10-13～2．0×10’¨mol·L～．thelinearregressioncoefficient1基于碳纳米材料检测肿瘤标志物的研究was0．9968andthedetectionlimitwas5．0×10‘13m

7、ol／L．Therelatix，estandarddex，iationfRSD)of8．7％hadbeenobtainedby10timesparalleldetectionfortheconcentrationof1．0×10一¨m01．L～targetDNA．Inthechapterfour,theglutathione(GSH)ofK562cellwerequantitativeanalysisbyconstructcarbonnanot