金黄色葡萄球菌耐药谱分型、pfge分型和hvr-pcr分型的比较分析

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1、ComparisonamongtheMethodsofDrugResistanceTyping，PFGETypingandHVR-PCRTypingofStaph3，lococcusaureusABSTRACT[Objective]TounderstandthedrugresistanceofStaphylococcusaureusinShenzhenPeople’sHospital．Applyingthreekindsoftypingmethods(DrugResistancetyping，PFGEtypi

2、ngandHVR·PCRtyping)tostudyandcomparethetypingresultsofStaphylococcusaareusstrains；PFGEandHVP—PCRtypingmethodswereperformedtosetupStaph)7lococcusaureuselectrophorograms；AnalyzingthegenehomologytoknowabouttheepidemicpresentsituationbyspecializedFingerprinting

3、IIsoftware．ToprovideaguildanceforreducingandcontrollingnosocomialinfectioncausedbytheStaphylococcusaureusstrainsinShenzhenPeople’sHospital．【Methods]1，ThecollectionofStaph)7lococcusaureusclinicalstrainsAccordingtotheanalysisinrecord，wecollectseparatedStaphyl

4、ococcusauFeusstrainsinstorefrompatients’specimeninShenzhenPeople’SHospitalfromJune2004toApril2005．NosameStaphylococcusaureusstrainwascollectedfromthesamepatient．2．DragresistancetypingandtheanalysisofthenosocomialinfectionTheantibioticsusceptibilitytests(AST

5、lwerecaniedoutfor18commonkindsofantibioticsbytheK—BmethodaccordingtotheStandardofNationalStandardizationCommitteeofClinicalLaboratory(NCCLS)in2004．AccordingtotheASTresults，Staph)，lococcusaureuscouldbedividedintomethicillin·resistantStaphylococcusaureus(MRSA

6、)andmethicillin-susceptibleStaphylococcusaureus(MSSA)groups．TomakeatypingofMRSAandMSSArespectivelyandidentifythenosocomialinfectionstrains，thencomparetheepidemicstatusofMRSAandMSSAinthehospital．3．Pulsed-fieldGelElectrophoresis(PFGE)typingStaphylococcusaureu

7、swereconductedwiththeprocessofdissolvinggerms，．3．deproteinizating，digesting，andthenbeingelectrophoresedinthesystemofBIO-PADGenePath’⋯．ThegenehomologyofPFGEprofileswasanalysedbytheFingerprintingIIsoftware，thentocomparetheresultsbetweenthePFGEtypinganddrugres

8、istancetypinginaccordanceofMSSAandMRSA．4．MecA-associatedhyper-variableregionpolymerasechainreaction(HVR—PCR)typingToextractthenucleicacidofStaphylococcusauFelg$，anddesignthespecificprimerformecA-associ