利用snp标记构建油菜品种指纹图谱与定位下卷叶性状基因的分析

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1、利用SNP标记构建油菜品种指纹图谱及定位下卷叶性状基因的研究differentenvironmentmighthavecertaininfluencetothegeneticrelationshipamongvarieties．(3)Thecomparisonbetweenf'mgerprintingsbyusingoftwokindsofmarkers：9fingerpfintingsofrapeseedvarietieswereestablishedbySNPandSSRmarkers，thegeneticsim

2、ilaritycoefficientandthecorrelationcoefficiembetweenthetwokindswerecalculated．ThegeneticsimilaritycoefficientwasO．3269(p-values=0．0517)，suggestedthatthetwomethodwereconsistent、历廿1eachother．Nosignificantdifferencehasbeenshownbetweenthedendrogrambyclusteranalysis

3、basedontwomarkersfor9rapeseedvarieties．Therewere52127SNPmarkersandonly40pairsofSSRprimers，therefore，thegeneticsimilaritycoefficientbyuseofSNPmarkerwasmoreaccurateandtheresultsweremorereliable．2．Mappingofthedown—curlyleafmutantgene．AnF2segregatingpopulationhadbe

4、enestablishedbasingonthedown—curlyleafmutantM423obtainedfromthemutantlibraryinourlaboratory．Therewere266down—curlyleafplantsand78normalplantsinthispopulation．Theseparationratioofthe．F2Wasconsistent谢tllthe3：1ratioofMendelianinheritancethroughChi-SquareTest，sugge

5、stedthatthedown—curlyleafmutationgenewasasingledominantgene．BySNPmarkeridentification，80individualsampleswererandomlyselectedforDNAextractionusingtheCTABmethod，fromwhichtheSNPmarkerdatawasobtained．10013polymorphicmarkerswereobtainedamongthe52127SNPmarkers．16SNP

6、markerswerefoundtoberelatedwiththemutanttraitthroughChi—SquareTestbyuseofthedataofSNPmarkers．Theresultsprovidedafoundationforthefinemappingofthedown—curlyleafmutantgeneinM423．Keywords：SNP；SSR；fingerprinting；down—curlyleaf；mapping；BrassicaNapusL．IVIULPAFLPRAPDSS

7、RSNPSDSPAGEPCREDTATEMEDCTABdNTPTris．妓UPGMAEMSASOTGGEDGGESSCPDHPLC缩略词表RestrictionFragmentLengthPolymorphismAmplifiedFragmentLengthPolymorphismRandomAmplifiedPolymorphicDNASimpleSequenceRepeatsSingleNucleotidePolymorphismSodiumDodecylSulfatePolyacrylamidegelElect

8、rophoresisPolymeraseChainReactionEthyleneDiamineTetraaceticAcidTetramethylethylenediamineCetrimoniunlBromideDeoxy—ribonucleosidetriphosphateTris(Hydroxymethyl)aminomethaneAm