欢迎来到天天文库
浏览记录
ID:32232198
大小:1.83 MB
页数:36页
时间:2019-02-01
《百令胶囊对糖尿病肾病大鼠肾组织足细胞nephrin表达的研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、山西医科大学硕士学位论文百令胶囊对糖尿病肾病大鼠肾组织足细胞Nephrin表达的研究摘要目的探讨百令胶囊对糖尿病肾病(DN)大鼠肾组织足细胞N印晡n表达的影响。方法清洁级雄性wistar大鼠40只,体重180~2009,随机分为正常对照组、DN组、百令胶囊组及贝那普利组。制作DN实验大鼠模型后,百令胶囊组及贝那普利组分别给予百令胶囊(2.59/l【g·d)及贝那普利(10m眺g·d)灌胃,实验周期12周。12周后观察各组血糖(BS)、24h尿蛋白定量、肾重/体重(KW,BW)、血肌酐(Scr)、尿素氮(BUN)、肌酐清除率(Ccr),取肾组织进行PAS、Masson染色,光镜观
2、察肾脏病理形态学变化。电镜观察肾小球基底膜厚度变化、足细胞数目变化及足突融合情况。采用免疫组化和ImPCR观察肾组织nepMn蛋白及基因表达的变化。结果与正常对照组相比,DN组大鼠BS、24h尿蛋白定量、Scr、BUN、KW/BW、肾小球硬化指数和肾小管损伤指数均显著上升(尸<0.05),Ccr显著下降(P0.05)。与正常对照组相比,DN组大鼠肾组织足细胞Nep¨n表达明显下调(P3、普利组N印¨n表达明显上调,与DN组比较,有显著性差异(P0.05)。光镜下DN组大鼠与正常组大鼠相比肾小球系膜基质增多,系膜区增宽;电镜下DN组大鼠肾小球基底膜显著增厚,足细胞排列紊乱,数目减少,足突融合;经治疗后与DN组比较,百令胶囊组及贝那普利组细胞外基质明显减少,系膜区增宽程度减轻,足细胞数目增多,足突融合明显减少,基底膜增厚程度减轻(均PO.05)。结论百令胶囊对DN大鼠具有一定的肾脏保护作用,其机制可能与上调肾组织足细胞N印hrin表达有关。关键词百令胶囊糖尿病4、肾病N印城n山西医科大学硕士学位论文Ef.fectofBailingcapsuleontheexpressionofNephrininthekidneyoftheratswithdiabeticnephropathyAbstractobjec晰eToinVestigatemeeff如ofBailingcapsuleonmecXpressionofn印嫡nin舭kidIlcyoftlleratswimdiabeticn印hropatlly.MethodsThemodelsofdiabeticnephropamyill眺wereestablishedbyinje“ngs仃eptoz5、otoc:inintoabdomeIl,40malespeCi6cpathog胁-丘优(SPF)WistarratsweremdomlydiVidcdinto4groups:no彻alcon们l舯up(n:lO),diab舐cn印hropathy印up(n=7),Bailingcapsule仃eatedgroup(n=8)a11db锄az印ril仃eatedgroup(n-7).111eratsinBailingcapsule讯斌edgr01Jpwere西V朗BailillgcapSule(2.59/l(g·d),锄dBellaz印ril骶ated黟oupwere西VeIlB锄6、钇印riltablet(10IIlg/l(g·d)缸ou曲iIl仃agaS仃icadminis蚴iononcedaily衙12weeks.Tllefatsin铭ch黟oupwererespectivelysa翻矗cedl2weeksaRermodelsestablished.B100dsugar(BS),kidIleywei曲泊odywei曲t(KW/BW),24h嘶mryprotein(24hUpr),Senlmcreatinine(S呻,B100durea11i仃DgerI(BUN),锄dCreatinineclear锄Cerate(C呻wereobseⅣcd.PASand7、MaSsonstainingwaSusedt0obseⅣemo印holo百calchaIlgesofrellaltissue.Immlnlol11istoch哪isny锄dI玎-PCRwereuscdtocatchtheproteinandmRNAeXpressionofN印hrinin佗naltissue.ResultsCompal司w油nomalcon仃olgroup,BS,KW,BW,24hUpr,C%aIld彻lal6broticlesiomdet甜oratedsi嘶6caIlⅡyi
3、普利组N印¨n表达明显上调,与DN组比较,有显著性差异(P0.05)。光镜下DN组大鼠与正常组大鼠相比肾小球系膜基质增多,系膜区增宽;电镜下DN组大鼠肾小球基底膜显著增厚,足细胞排列紊乱,数目减少,足突融合;经治疗后与DN组比较,百令胶囊组及贝那普利组细胞外基质明显减少,系膜区增宽程度减轻,足细胞数目增多,足突融合明显减少,基底膜增厚程度减轻(均PO.05)。结论百令胶囊对DN大鼠具有一定的肾脏保护作用,其机制可能与上调肾组织足细胞N印hrin表达有关。关键词百令胶囊糖尿病
4、肾病N印城n山西医科大学硕士学位论文Ef.fectofBailingcapsuleontheexpressionofNephrininthekidneyoftheratswithdiabeticnephropathyAbstractobjec晰eToinVestigatemeeff如ofBailingcapsuleonmecXpressionofn印嫡nin舭kidIlcyoftlleratswimdiabeticn印hropatlly.MethodsThemodelsofdiabeticnephropamyill眺wereestablishedbyinje“ngs仃eptoz
5、otoc:inintoabdomeIl,40malespeCi6cpathog胁-丘优(SPF)WistarratsweremdomlydiVidcdinto4groups:no彻alcon们l舯up(n:lO),diab舐cn印hropathy印up(n=7),Bailingcapsule仃eatedgroup(n=8)a11db锄az印ril仃eatedgroup(n-7).111eratsinBailingcapsule讯斌edgr01Jpwere西V朗BailillgcapSule(2.59/l(g·d),锄dBellaz印ril骶ated黟oupwere西VeIlB锄
6、钇印riltablet(10IIlg/l(g·d)缸ou曲iIl仃agaS仃icadminis蚴iononcedaily衙12weeks.Tllefatsin铭ch黟oupwererespectivelysa翻矗cedl2weeksaRermodelsestablished.B100dsugar(BS),kidIleywei曲泊odywei曲t(KW/BW),24h嘶mryprotein(24hUpr),Senlmcreatinine(S呻,B100durea11i仃DgerI(BUN),锄dCreatinineclear锄Cerate(C呻wereobseⅣcd.PASand
7、MaSsonstainingwaSusedt0obseⅣemo印holo百calchaIlgesofrellaltissue.Immlnlol11istoch哪isny锄dI玎-PCRwereuscdtocatchtheproteinandmRNAeXpressionofN印hrinin佗naltissue.ResultsCompal司w油nomalcon仃olgroup,BS,KW,BW,24hUpr,C%aIld彻lal6broticlesiomdet甜oratedsi嘶6caIlⅡyi
此文档下载收益归作者所有