猪c3d分子佐剂增强prrsvgp5核酸疫苗免疫效果的的研究

《猪c3d分子佐剂增强prrsvgp5核酸疫苗免疫效果的的研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、猪C3d分子佐剂增强PRRSVGP5核酸疫苗免疫效果的研究了pcDNA3.1-GP5-p28.n（2、4、6）重组质粒。大量提取重组质粒，转染Marc145细胞，经间接免疫荧光证明重组质粒能在细胞内表达。重组质粒免疫小鼠，通过ELISA试剂盒检测小鼠体内抗体、IL-4和IFN-γ的水平，结果显示，抗体效价、IL-4和IFN-γ含量均比对照组高，且以pcDNA3.1-GP5-P28.6组的效果最好，与空白对照及PCDNA3.1-GP5组比较差异均显著（P<0.05）。证明该质粒可刺激机体产生较高的体液免疫和细胞免疫水平，补体C3d-P28分子佐剂能显著增强PR

2、RSVGP5基因的免疫效果。关键词：PRRSV；C3d-p28佐剂；GP5基因疫苗；免疫效果2山东农业大学硕士学位论文StudyonthePigC3dmolecularadjuvantenhancingtheeffectofPRRSVGP5DNAvaccineAbstractGeneticimmunizationorDNAvaccinationhasinitiatedaneweraofvaccineresearch.ThetechnologyinvolvestheinoculationofplasmidDNAintoalivinghosttoelicitan

3、immuneresponsetoaproteinencodedontheplasmid.ThepotentialadvantagesofDNAvaccinesincludetheinductionofcellularandhumoralimmuneresponses，flexiblegeneticdesign，lackofinfectionrisk，stabilityofreagents，andtherelativelylowcostofproductioninamicrobialhost.Howeverthegenevaccinecouldnotprovo

4、catehightiterandlong-lastingimmuneresponse.SeveralapproacheswereexploredtoenhancetheimmunityofDNAvaccinessuchasusingmolecularadjuvant.ComplementC3isthecorefactoroftheclassicalpathway，alternativepathwayandlectinpathway.CleavagesofC3haveextensiveimmunefunction.C3disthefinalcleavagepr

5、oductofC3.It’sbeenthoughttobetheleastcleavagewhichcan’tbeendigestedbyproteaseandcancovalentlinkedtotheantigen.ItcanattachtotheCR2whichisonthesurfaceofantigen-presentingcells(APC),soitcutsdowntheactivationthresholdoflymphocyteandelevatestheimmunityoftheantigen.C3dhasbeendefinedtobea

6、neffcctivemolecularadjuvant.PorcineReproductiveandRespiratorySyndromeVirus(PRRSV)wasthepathogenyofPorcineReproductiveandRespiratorySyndrome(PRRS)representedbyabortionofsewandrespiratoryobstacleofpiglets.Foritsinfectiousnessaswellastheimmunerepressionandintercurrentbroughttopigs，PRR

7、SVcausedhugeeconomiclosstopig’sfeedingindustry.Moreover，themutantsofPRRSVgenesincreasethedifficultytocontroltheillness.Glycoprotein5(GP5)istheprimarystructuralproteinofPRRSV.Itshowsthefunctionofcombiningthevirustothereceptoronthehostcellaswellasinducingtheapoptosisofthecellandnovel

8、vaccinewasdesignedaccordin