番茄np24基因的遗传转化

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1、中国农学通报2018,34(13):57-63ChineseChineseAgriculturalScienceBulletinAgriculturalScienceBulletin番茄NP24基因的遗传转化陈修花，赵敬一，张喜春（北京农学院植物科学技术学院农业应用新技术北京市重点实验室，北京102206）摘要：本研究旨在通过将类甜蛋白基因NP24导入番茄，提高番茄品种的抗病能力。通过对‘京乐502’‘、耐运2000’以及‘Bolgogragsky’3个番茄品种进行抗病性的鉴定及防御酶活性测定，选取抗病性最弱的番茄品种‘Bolgogragsky’，采用农杆菌介导法将类甜蛋白基因N

2、P24导入番茄。接种晚疫病7天后测得‘Bolgogragsky’‘、耐运2000’和‘京乐502’病情指数分别为81.7、48.7、16.0。‘Bolgogragsky’POD酶活性在第1天达到高峰‘，京乐502’‘、耐运2000’在第5天到达最高峰，但‘京乐502’的酶活性一直较其他2组高。PAL酶活性先升高后降低‘，京乐502’升得最快降得最慢‘，Bolgogragsky’升得最慢降得最快‘。京乐502’SOD酶活性在0~5天升高而后降低‘，耐运2000’‘、Bolgogragsky’接种后第1天SOD酶活性明显降低，后续第3、5、7天在波动中上升。实验结果表明接种晚疫病菌后

3、3个品种番茄的POD、PAL酶活性均高于对照，且酶活性与病情指数呈负相关；而处理组SOD酶活性均较对照组有所降低。此外，经PCR验证NP24基因已经整合到番茄‘Bolgogragsky’基因组中。关键词：番茄；抗病；防御酶；类甜蛋白；遗传转化中图分类号：S641.2文献标志码：A论文编号：casb17110012GeneticTransformationofNP24GeneinTomatoChenXiuhua,ZhaoJingyi,ZhangXichun(CollegeofPlantScienceandTechnology,BeijingUniversityofAgricultu

4、re,Beijing102206)Abstract:Thestudyaimsatimprovingthediseaseresistanceoftomatovarietiesbyinducinghaumatin-likeproteinsgeneNP24intotomato.Throughtheidentificationofdiseaseresistanceandtheactivityofdefensiveenzymeof‘Jingle502’,‘Naiyun2000’and‘Bolgogragsky’,theweakestdisease-resistancevariety‘Bol

5、gogragsky’wasselectedtotransformtheNP24genewiththeAgrobacterium-mediatedmethod.Thediseaseindexof‘Bolgogragsky’,‘Naiyun2000’and‘Jingle502’after7daysofPhytophthorainfestansinoculationwas81.7,48.7and16.0,respectively.TheactivityofPODenzymein‘Bolgogragsky’reachedthethpeakonthefirstday,whilethatof

6、‘Naiyun2000’and‘Jingle502’peakedonthe5day,but‘Jingle502’hadconsistentlyhigherenzymeactivitythanthatoftheothertwovarieties.TheactivityofPALenzymeincreasedfirstandthendecreased,‘Jingle502’showedthefastestincreaseandtheslowestdecrease.‘Bolgogragsky’showedthelowestincreaseandthefastestdecrease.Th

7、eactivityofSODenzymein‘Jingleth502’increasedonthe0-5dayandthendecreased,buttheactivityofSODenzymein‘Bolgogragsky’and‘Naiyun2000’significantlydecreasedonthefirstdayafterinoculation,anddisplayedanincreasewithrdththfluctuationonthe3,5and7day.The