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1、1706王星敏等/酶解破壁促进废次烟叶中茄尼醇溶浸生物工程学报ChineseJournalofBiotechnologyNovember25,2013,29(11):1706−1710http://journals.im.ac.cn/cjbcn©2013ChinJBiotech,Allrightsreserved生物育种与工艺优化酶解破壁促进废次烟叶中茄尼醇溶浸王星敏，张渝文，张桂芝，殷钟意重庆工商大学环境与生物工程学院，重庆400067王星敏,张渝文,张桂芝,等.酶解破壁促进废次烟叶中茄尼醇溶浸.生物工程学报,2013,29(11):1706−1710.WangXM,Z
2、hangYW,ZhangGZ,etal.Improvedextractionofsolanesolfromtobaccowastebyenzymaticcellwallbreaking.ChinJBiotech,2013,29(11):1706−1710.摘要:协同应用纤维素酶和木质素酶催化降解废次烟叶，探讨清洁高效的酶解破壁效应及浸提茄尼醇工艺条件。结果发现复配酶催化裂解溶浸茄尼醇效果明显优于单一酶，酶解时间、温度、pH值以及酶投加量等条件均影响酶破壁浸提茄尼醇能效。结果表明，采用纤维素酶：木质素酶酶活比15∶1(U/U)的复配酶，在体积为5倍烟草质量的水介质环境中，当
3、复配酶投加量为175U/g，水浴温度40℃，pH为6时，催化酶解烟叶8h后，茄尼醇溶浸浓度可达0.33g/L。在此条件下，茄尼醇平均提取率可达96.53％，是化学回流浸提方法的1.68倍。该方法为有效提取废次烟草中茄尼醇提供了一种新途径。关键词:酶促反应，茄尼醇，废次烟草，破壁，提取ImprovedextractionofsolanesolfromtobaccowastebyenzymaticcellwallbreakingXingminWang,YuwenZhang,GuizhiZhang,andZhongyiYinCollegeofEnvironmentalandBi
4、ologicalEngineering,ChongqingTechnologyandBusinessUniversity,Chongqing400067,ChinaAbstract:Toimprovetheextractionofsolanesolfromtobaccowaste,wedevelopedanenzymaticcellwall-breakingprocesswithcombinedcellulaseandligninase.Theeffectsofreactiontime,temperature,pHandenzyme/substrateratiowered
5、etermined.Theresultsshowthatthecatalyticeffectwasbetterthaneithersingleenzymewhentheratioofcellulasetoligninasewas15:1(U/U).Undertheoptimizedconditionsof175U/g(enzymes/substrate),tobaccotowater1:5(W/W),Received:January23,2013;Accepted:March1,2013Supportedby:KeyTechnologiesResearchandDevel
6、opmentProgramofChongqing(No.cstc2011AB7060),ProgramforChongqingInnovativeResearchTeamDevelopmentinUniversity(No.KJTD201020).Correspondingauthor:XingminWang.Tel:+86-13-527536325;Fax:+86-23-62768501;E-mail:wang_chem@tom.com重庆市科技攻关项目(No.cstc2011AB7060)，重庆市教委资源化学与利用项目(No.KJTD201020)资助。网络出版时间:
7、2013-07-10网络出版地址:http://www.cnki.net/kcms/detail/11.1998.Q.20130710.1613.001.html王星敏等/酶解破壁促进废次烟叶中茄尼醇溶浸1707temperature40°CandpH6.0,theconcentrationofsolanesolinthesolutioncouldreach0.33g/Lafter8h.Andtheaverageleachingratereached96.53%whichwas1.68timesoftheextractionm
