l-name对隐睾下降固定术后生精细胞凋亡的影响

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1、normalsalineandL-NAME(50mg/Kg)accordingly.Thebloodsampleswerecollectedfromthetailafterinjectiontwohoursonthe12thday.Theserumwerecollectedandstoredat–80℃forsubsequentdetection.SerumNOconcentrationwasmeasuredbyGreissmethod.TheactivityofNOSinserumwasmeasuredbycolorimetricmethod.Theratsweresacrific

2、edonthe24thdayafterthesecondoperation.Themorphologyofspermatogenicepitheliuminratsofeverygroupwasobservedwithlightmicroscopy.ThelevelofspermatogeniccellapoptosisintheunilateralcryptorchidismandorchidopexyratswereevaluatedbyFCMandTUNEL.TheproteinexpressionlevelofeNOSandiNOSintestesweredetectedby

3、Westernblotandimmunohistochemicalassayaccordingly.Results:Thelevelofspermatogeniccellapoptosis,serumNOconcentrationandtheactivityofNOSinratscryptorchidismwerehigherthantheratsorchidopexybuttheweightoftesteswerelighterthantheratsorchidopexy.AdministrationofL-NAMEcoulddecreaseserumNOconcentration

4、andspermatogeniccellapoptosisinratsexperimentalorchidopexy,anditcouldsignificantlyinhibitweightloseoftheorchidopexiedtestes.TheactivityofNOSwasattenuatedsignificantlybyL-NAME.TheproteinexpressionlevelofeNOSandiNOSinthetestesoftheshamoperationgroupwasthelowestinthefourgroups.TheexpressionofeNOSa

5、ndiNOSinthespermatogenicepitheliumwerefeebleintheshamoperationgroupbutstronginotherthreegroupsbyimmunohistochemicalassay.8Conclusions:1.Theimprovementofspermatogenesisisrelatetothereductionofspermatogeniccellapoptosisinratsexperimentalorchidopexy.2.NOistheimportantbiologyactivegeneofexcessapopt

6、osisinspermatogeniccellinratsexperimentalorchidopexy.3.L-NAMEcanreducetheproductionofNOandspermatogeniccellapoptosis,itshowsthattheinhibitorofNOScanpromotetheenhancementofspermatogeniccapabilityinratsexperimentalorchidopexy.Postgraduate:ShiJinfeng(HumananatomyandHisto-embryology)Directedby:HeLi

7、ping,professorKeywords:L-NAME;spermatogeniccell;apoptosis;orchidopexy9前言男性生殖生理及生殖生物学是一门新兴的边缘学科，由于男科学的研究起步较晚，许多男科疾病的发生、发展规律至今尚未完全研究清楚，很多男性常见疾病如男性不育的治疗方法及效果仍然不尽人意。WHO调查显示，由于社会压力及环境因素的影响，男性精液质量明显下降，不育夫妇中由男方因素引起者占40%以上。研究表明许多特发性男性不育患者