小麦抗白粉病基因pm40的分子标记定位与同源共线序列片段分析

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1、万方数据2．PreviousreportedSSRmarkerslinkedwithPr040werefirstlyusedtotestforpolymorphisminpresentpopulation．Fourmarkers0(wmc335，Xgwm297，Xujmc364，m4’mc476)showedclearpolymorphismsandwerelinkedwithPm40．TwoadditionalmarkersC翔?mc662，Ⅸ彤，m43)outof87SSRmarkersmappedonchromosome7BSshowedp

2、olymorphismsandwerelinkedwithPr040．Theorderofthe6SSRmarkersonwheat7BSis炯，mc335，Ⅸ即，m297jXwmc662，m1’mc476．jn'’mc364．Xgwm43．ThegeneticdistancebetweenPm40andthemarkerswere0．58cM，0．74cM?0。24cM，0．37cM，O。33cMand0。86cM，respectively．ThePm40isflankedbyXwmc335andX卧，m297．3．Tosaturatethec

3、hromosomalregioncontainingthePr040locuswithmoremarkers，351EST-STSmarkersweredex，elopedaccordingtothewheatESTsmappedonchromosome7BS．Resultsshowedthat22markerswerepolymorphicandsevenwerelinkedwithPr040intheF2population．Eachmarkerlocussegregatedin1：2：1or3：1ratios．Alinkagemapflan

4、kingwheatchromosome7BSwasconstructedwithsixSSRmarkersandsevenEST-STSs．Pm40iSflankedbyBF291338andXwmc335atgeneticdistancesofO．z6cMand0．58cM。respectiveb7．Thegeneticmapspans6．181cMandtheaveragedistanceis0．44cMbetweenmarkers．4．ToaccuratelYcharacterizethecollinearitybetweenthePr04

5、0regionandthegenomicregionsofB14ach)podiumdistachy077．OryzasativajaponicaandSo?"ghumbicolor，threesequences(BE423064，BE446．559，BF291338)correspondingtothemappedwheatESTswereusedasqueriestoperformaBLASTsearchagainsttheBrachypodiumdistachyon．Or3zasat^，ajaponicaandSorghumbicolorg

6、enomesequences．ComparativegenomicanalysisestablishedthecollinearityofthePm40genomicregionwitha1．09Mbpgenomicregiononchromosome3inBrachypodiumdistach)，0t"7，a1．16Mbpgenomicregiononchromosome8inOryzasati．11ajaponicaanda1．62Nlbpgenomicregiononchromosome7inSorghumbicolor．5．Thehigh

7、densitygeneticmapofPm40iscapableofprovidingexcellentgeneticmarkersformarkerassistantslectionbreeding，itcanspeedupthebreedingprogress；ThePr040regionorthologouscolinersequenceonBrach3podiumdistach370n，Or3=asativajaponicaandSorghumbicolordiscoverTedinthisstud)"couldpalyanimporta

8、ntroleinseekingforcandidategeneandhomologouscloningKeywords：Wheat；Po