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ID:33211750
大小:7.98 MB
页数:49页
时间:2019-02-22
《甜瓜细菌性叶枯病胶体金免疫试纸条的研发与应用》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、万方数据ABSTRACTDEVELoPMENTANDAPPLICATIoNoFACoLLoIDALGoLDIMMUNoCHRoM已订oGRAPHICASSAyFoRDETECTIoNoFMUSKMELoNBACTERIALLEAFSPoTBLIGHTBacterialspotofmelonleavescausedbyPseudomonassyringaepv.1achrymansisdistributedworldwidelyandinflictsdifferentdegreesofdamage.Pseudomonassyringaepv.1a
2、chrymanshasbecomegreatthreattocucumberandmelonindustry.Bacterialfruitblotchisthemostimportantmelonseed—bornedisease,thepathogenisAcidovoraxcitrulli,whichisaworldwidequarantineddisease.MeloninfectedAcidovoraxcitrulliandPseudomonassyringaepv.1achrymans,thesymptomsareverysimila
3、rtoeachother.Itisdifficulttousetraditionalmethodsdistinctingbetweenthetwo.Forthisreason,ensuringtheexclusionofinfestedseedsbyapplyingtheeffective,commerciallyviableandconvenientdetectingtechnologiesiscrucialforthefoodindustry.Theresearchmethodwasusingintraperitonealimmunizat
4、ionimmunizedmice.ThecellfusiontechniquetoobtainhybridomacelllinesproducingmonoclonalantibodiesWaSsuccessful.Additionally,doubleantibodysandwichmodelusingtwodifferentmonoclonalantibodypreparationofimmunochromatographicstripWasachieved.Anantimuskmelonbacterialleafspotblightmon
5、oclonalantibody(b)fixedtonitrocellulosemembraneinthedetectionareaandanotherkindofantimuskmelonbacterialleafspotblightmonoclonalantibody(c)coupledtocolloidalgoldparticlesaschromogenicagent.Subsequentlytheplantsamplesolutionbycapillaryactionreactionwithantimuskmelonbacterialle
6、afspotblightantibodywasaddedtothedetectionarea,themuskmelonbacterialleafspotblightretentioninthedetectionareawhichappearedredgavepositiveresults.Inqualitativedetection.thedetectionlimitofmuskmelonbacterialWasdeterminedas2.5×103cfu/mL.SpecificityresultsshowedthatthisstripWasn
7、oCROSS—reactivity、ⅣimAcidovoraxcitrulli.III万方数据甜瓜细菌性叶枯病胶体金免疫试纸条的研发与应用andhadverystrongspecificity.InthequantRativedetection,thedetectionresultswerescannedbyamembranestripreader,astandardinhibitioncurveandcMibrmioncurvewereestablished,theregressionequationwasy=0.3922x一1.2638,掣
8、=0.9881.Therefor,thedevelopeddoubleantibodysandwichcolloidalgoldstripisanac
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