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ID:33995144
大小:7.98 MB
页数:49页
时间:2019-03-03
《甜瓜细菌性叶枯病胶体金免疫试纸条的研发与应用》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、万方数据ABSTRACTDEVELoPMENTANDAPPLICATIoNoFACoLLoIDALGoLDIMMUNoCHRoM已订oGRAPHICASSAyFoRDETECTIoNoFMUSKMELoNBACTERIALLEAFSPoTBLIGHTBacterialspotofmelonleavescausedbyPseudomonassyringaepv.1achrymansisdistributedworldwidelyandinflictsdifferentdegreesofdamage
2、.Pseudomonassyringaepv.1achrymanshasbecomegreatthreattocucumberandmelonindustry.Bacterialfruitblotchisthemostimportantmelonseed—bornedisease,thepathogenisAcidovoraxcitrulli,whichisaworldwidequarantineddisease.MeloninfectedAcidovoraxcitrulliandPseudom
3、onassyringaepv.1achrymans,thesymptomsareverysimilartoeachother.Itisdifficulttousetraditionalmethodsdistinctingbetweenthetwo.Forthisreason,ensuringtheexclusionofinfestedseedsbyapplyingtheeffective,commerciallyviableandconvenientdetectingtechnologiesis
4、crucialforthefoodindustry.Theresearchmethodwasusingintraperitonealimmunizationimmunizedmice.ThecellfusiontechniquetoobtainhybridomacelllinesproducingmonoclonalantibodiesWaSsuccessful.Additionally,doubleantibodysandwichmodelusingtwodifferentmonoclonal
5、antibodypreparationofimmunochromatographicstripWasachieved.Anantimuskmelonbacterialleafspotblightmonoclonalantibody(b)fixedtonitrocellulosemembraneinthedetectionareaandanotherkindofantimuskmelonbacterialleafspotblightmonoclonalantibody(c)coupledtocol
6、loidalgoldparticlesaschromogenicagent.Subsequentlytheplantsamplesolutionbycapillaryactionreactionwithantimuskmelonbacterialleafspotblightantibodywasaddedtothedetectionarea,themuskmelonbacterialleafspotblightretentioninthedetectionareawhichappearedred
7、gavepositiveresults.Inqualitativedetection.thedetectionlimitofmuskmelonbacterialWasdeterminedas2.5×103cfu/mL.SpecificityresultsshowedthatthisstripWasnoCROSS—reactivity、ⅣimAcidovoraxcitrulli.III万方数据甜瓜细菌性叶枯病胶体金免疫试纸条的研发与应用andhadverystrongspecificity.Int
8、hequantRativedetection,thedetectionresultswerescannedbyamembranestripreader,astandardinhibitioncurveandcMibrmioncurvewereestablished,theregressionequationwasy=0.3922x一1.2638,掣=0.9881.Therefor,thedevelopeddoubleantibodysandwichcolloidalgoldstripisanac
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