gjb2基因条件敲除小鼠耳蜗凋亡相关基因表达差异研究

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1、分类号：密级：单位代码：10092学号：20111019GJB2基因条件敲除小鼠耳蜗凋亡相关基因表达差异研究单位：河北北方学院基础医学院专业：病理学与病理生理学研究生姓名：崔小缓导师姓名及职称：张延平教授、金春亭教授研究起止日期：2013.4-2014.3提交日期：2014.3万方数据河北北方学院学位论文使用授权及知识产权归属承诺本学位论文在导师（或指导小组）的指导下，由本人独立完成。本学位论文研究所获的研究成果，其知识产权归河北北方学院所有。河北北方学院有权对本学位论文进行交流、公开和使用。导师签名：研究生签名：201

2、4年6月10日河北北方学院研究生学位论文独创性声明本论文是在导师指导下进行的研究工作及取得的研究成果，除了文中特别加以标注和致谢等内容外，文中不包含其他人已经发表或撰写的研究成果，指导教师对此进行了审定。本论文由本人独立撰写，文责自负。导师签名：研究生签名：2014年6月10日万方数据目录目录中文摘要····························································································1英文摘要···············

3、············································································3英文缩写···························································································6研究论文GJB2基因条件敲除小鼠耳蜗凋亡相关基因表达差异研究前言··························································

4、·································8材料与方法···························································································9结果···························································································15附图···································

5、························································19附表···························································································28讨论···························································································46结论·············

6、··············································································52参考文献···························································································53综述细胞凋亡与耳聋研究进展··················································58致谢···················

7、········································································76个人简历···························································································77I万方数据中文摘要GJB2基因条件敲除小鼠耳蜗凋亡相关基因表达差异研究摘要耳聋是导致人类言语交流障碍的主要疾病，在中国人中，GJB2基因突变导致的先天性耳聋大约为12.2%，在NSHL

8、未成年患者中GJB2基因突变的阳性检出率高达20.1%，该基因突变是导致人类非综合征性聋（nonsyndromichearingloss，NSHL）的主要原因，研究其致病机Pax2Cre制意义重大。研究表明GJB2基因条件敲除小鼠（cCx26小鼠）出生后出现耳蜗细胞缺失，凋亡可能是GJB2基因敲除后耳蜗细胞缺失的主要