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时间:2019-02-27
《胞壁酰二肽对人牙髓细胞分化影响的研究》由会员上传分享,免费在线阅读,更多相关内容在工程资料-天天文库。
1、万方数据·412·生堡旦堕匡堂苤盍!Q!!生!旦筮箜鲞筮!塑坠i!』!鲤型!!:』!!!垫!!:坠!:堑:盟璺!.牙体牙髓病学研究.胞壁酰二肽对人牙髓细胞分化影响的研究罗传霞张莉宋智秦伟林正梅【摘要】目的探讨天然免疫受体核苷酸结合寡聚化结构域蛋白2(nucleotide-bindingoligomerizationdomain-2,NOD-2)在深龋牙髓组织中的表达及NOD.2特异性配体胞壁酰二肽(muramyldipeptide,MDP)对人牙髓细胞分化的影响。方法实时荧光定量聚合酶链反应(PCR)
2、法检测深龋及健康人牙髓组织中NOD.2表达差异;1mg/LMDP刺激体外培养的人牙髓细胞0、2、6、12、24h,实时荧光定量PCR、蛋白质免疫印迹法及免疫荧光检测NOD.2基因及蛋白表达;不同质量浓度MDP刺激人牙髓细胞24h,蛋白质免疫印迹法检测24h后牙本质涎蛋白(dentinsialoprotein,DSP)的表达;o.1mg/LMDP作用于人牙髓细胞0、2、6、12、24h,检测不同时间点牙本质涎磷蛋白(sialophosphoprotein,DSPP)、骨钙蛋白mRNA及骨桥蛋白的表达。结
3、果深龋牙髓组织中NOD-2mRNA相对表达量为(0.26104-0.0824),较健康牙髓组织(0.00244-0.0002)显著增高(P4、2表达升高,MDP与人牙髓细胞的分化相关。【关键词】Nod2信号接头蛋白质;乙酰胞壁酰.丙氨酸一异谷酰胺;细胞分化;牙髓细胞EffectsofmuramyldipeptideonthedifferentiationofhumandentalpulpcellsLUOChuan-xia,ZHANGLi,SONGz“,QINWei。uNZheng—mei‘.‘DepartmentofOperativeDentistryandEndodontics.GuanghuaSchoolandHospitalofSto5、matology&InstituteofSwmawIcogwalResearch,SunYat—senUnivers渺,Guangzhou510055,ChinaCorrespondingauthor:uNZheng-mei。Email:linzhm@mailsystae机cn。Tel:0086-20-83741753【Abstract】objectiveToinvestigatethenucleotide—bindingoligomerizationdomain-2(NOD-2)geneexpres6、sionindeepcariesandtheeffectsofNOD-2agonistmuramyldipeptide(MDP)onthedifferentiationofhumandentalpulpcells(hDPC).MethodsNOD.2genelevelindeepcariesandhealthypulptissuewasdeterminedbyreal-timequantimtivepolymerasechainreaction(realtime—PCR).ReMtime—PCR.We7、sternblottingandimmunofluorescencewereperformedtoevaluateNOD-2geneandproteinexpression.Dentinsialoprotein(DSP)proteinlevelwasassessedwhenhDPCwerechallengedbydifferentconcentrationsofMDPfor24hours,andsialophosphoprotein(DSPP),osteocalcin(OCN)mRNAandosteo8、pontin(OPN)proteinlevelweredetectedatdifferenttimepointsafterincubationwith0.1me-/LMDP.ResultsNOD-2mRNAlevelWashilgherinpulptissueofdeepcaries(0.26104-0.0824)thanthatinhealthycontrols(O.00244-0.0002)。P<0.05.TheexpressionofNOD-2ge
4、2表达升高,MDP与人牙髓细胞的分化相关。【关键词】Nod2信号接头蛋白质;乙酰胞壁酰.丙氨酸一异谷酰胺;细胞分化;牙髓细胞EffectsofmuramyldipeptideonthedifferentiationofhumandentalpulpcellsLUOChuan-xia,ZHANGLi,SONGz“,QINWei。uNZheng—mei‘.‘DepartmentofOperativeDentistryandEndodontics.GuanghuaSchoolandHospitalofSto
5、matology&InstituteofSwmawIcogwalResearch,SunYat—senUnivers渺,Guangzhou510055,ChinaCorrespondingauthor:uNZheng-mei。Email:linzhm@mailsystae机cn。Tel:0086-20-83741753【Abstract】objectiveToinvestigatethenucleotide—bindingoligomerizationdomain-2(NOD-2)geneexpres
6、sionindeepcariesandtheeffectsofNOD-2agonistmuramyldipeptide(MDP)onthedifferentiationofhumandentalpulpcells(hDPC).MethodsNOD.2genelevelindeepcariesandhealthypulptissuewasdeterminedbyreal-timequantimtivepolymerasechainreaction(realtime—PCR).ReMtime—PCR.We
7、sternblottingandimmunofluorescencewereperformedtoevaluateNOD-2geneandproteinexpression.Dentinsialoprotein(DSP)proteinlevelwasassessedwhenhDPCwerechallengedbydifferentconcentrationsofMDPfor24hours,andsialophosphoprotein(DSPP),osteocalcin(OCN)mRNAandosteo
8、pontin(OPN)proteinlevelweredetectedatdifferenttimepointsafterincubationwith0.1me-/LMDP.ResultsNOD-2mRNAlevelWashilgherinpulptissueofdeepcaries(0.26104-0.0824)thanthatinhealthycontrols(O.00244-0.0002)。P<0.05.TheexpressionofNOD-2ge
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