大肠埃希菌多重耐药外排泵acrab-tolc主动外排机制的研究

《大肠埃希菌多重耐药外排泵acrab-tolc主动外排机制的研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、天津医科大学硕士学位论文3，Amplificationofeffluxpumpgeneacra／acrb：applicationofpolymerasechainreaction(PCR)amplificationof90clinicalisolatedstrainsofE。coliofaera／aerbgenefragments，PCRproductssendBeOinghuadatechnologycompany，usethepositiveandnegativetwo—wayDNAsequenceanalysis．4，CCCPinhib

2、itoryexperiment：select10multipledrug-resistantE．colistrainsforCCCPinhibitoryexperiments，totracethebrothdilutionmethodandpumpinhibitorsCCCP(finalconcentrationof100umogL)beforeandaftermultipledrug—resistantstrainsoffivekindsofantimicrobialminimumbacteriostaticconcentration(MI

3、C)．Results：1，Thetotalcollectionof90strainsofe．coli，37strainsofisolatedbacteriafromurethra，36fromintestinal，5fromTherespiratorytract，1lfromothersecretions(blood，ascites，etc．)．2，invitroantibioticssensitiveexperimentresults：90strainsofE．colion6kindsofantimicrobialdrugsshoweddi

4、fferentdegreesofresistance，resistanceratefromhilghtolow：ciprofloxacinas67％，levofloxacin61％，gentamicin53％，cefotaxime50％，aztreonam20％，amikacin10％．Drugresistancepatternof30doubledrug-resistantstrains(33．3％)，26multipledrug-resistantstrains(28．9％)，22singledrugresistantstrains(24

5、．4％)，12wholesensitivestrains12(13．3％)．3，acra／acrbamplificationandsequencingresults：90clinicalisolatedE．colistrains，55(61．1％)arevisibleof567bp(acra)and516bp(acrb)fragmentsamplified．StaphylococcusaureusATCC25923didnotseethefragmentsamplified．Clinicalstrain246ofacrageneandclin

6、icalstrainsb716ofacrbgeneamplificationareseletedforpositiveandnegativetwo—wayDNAsequenceanalysis，thesequencingresultshitchesinGeneBankdatabaserOWShomologycomparisonafterconfirmed：clinicalstrains246，acragenehomologyof98％，theclinicalstrainsb716，acrbgenehomologyof99％．Themulti—

7、resistantgroupof22strainsofacrapositive(84．6％)，21strainsofacrbpositive(80．8％)；Twodrugresistantgroupof16strainsofacratestedpositive(53．3％)，17strainsofacrbtestedpositive(56．7％)；Singledrugresistantgroupof12strainsofacrapositive(54．5％)，13strainsofacrbV天津医科大学硕士学位论文positive(59．1％

8、)；Allsensitivegroupof5strainsofacraand4strainsofacrb(33．3％)werepositive(41．7％)，．So