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1、IdentificationofastrainofisolatedNannochloropsistheeffectonfattyacidunderdifferentpHAbstract:AstrainofmicroalgaisolatedfrommangrovesedimentofHainanwasidentifiedtobeNannochloropsis.maritima.Theeffectofdifferentfactors(nutrientmedium,nitrogenresourceandcorrespondednitrogencon
2、centration,phosphorusconcentration,salinity,pHandtemperature)onthegrowthofseparatedNannochloropsismaritimawerecompared.Resultsshowedthatthestrainofisolatedmicroalgagrewrelativelybetteratnutrientmediumf/2withsalinity30,74.8mg/LNaNO3(Nmassconcentrationwas1.705mg/L),6.6mg/LKH2
3、PO4(Pmassconcentrationwas1.705mg/L),pH6.0.Culturedat26℃,itscelldensitycouldbeup4to5590×10/mLduringsevendays'growthandrelativegrowthratewas0.7861.ComparisonwithNannochloropsisoculataonrelatedreports,theisolatedmicroalgashouldbeafast-growingmicroalgawithendurancetolownitrogen
4、andsourishenvironment.pHhasanobviouseffectonthegrowth,totallipidcontentofmarinemicroalgaNannochloropsismaritimaResultsshowedthatahighgrowthrateofthealgawasobtainedundertherangeofpH5.0-9.0,butthegrowthwasveryslowlyatpH9.0.underdifferentpHconditions,theamountoffattyacidschang
5、etherearecertainrulestofollow,Thetotallipidofthespeciesincreasedgraduallyasthecultureagedexceptforaslightreductionintheearlyexponentialphase.AnalysisdefoundthatherelativefluorescenceintensityofNannochloropsismaritimafrompH5topH9wasatrendofdecreasing,therelativefluorescencei
6、ntensitywhenpH5wastopandpH9relativefluorescenceintensityofthelowest.pHalsohasanobviouseffectonfattyacidcompositionofmarinemicroalgaNannochloropsismaritima,Resultsshowedthatthefattyacidundertheconditionoftheacidhadmorekindsthanthekindsoffattyacidinalkalineconditions.AndSatur
7、atedfattyacidswastheThehighestlevel,C16:0wasthehighestproportionoffattyacidspecies,ThenwasC14:0,WiththeincreaseofpHgradient,C14:0contentalsohasatendencytorise,weknowthathighpHcultureconditionsmoreconduciveIIItotheaccumulationofC14:0.C16:0wasthehighestproportionoffattyacidsp
8、ecies,ThenwasC14:0.UnderthecondionofpH5thecontentofC16:0wasashighas35.76497%,butat
