实验室常用缓冲液的配置方法

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1、标准缓冲液pH值与温度对照表温度℃四草酸氢钾0.05M邻苯二甲酸氢钾0.05M混合磷酸盐0.025M硼砂0.01M51.674.006.959.39101.674.006.029.33151.674.006.909.28201.684.006.889.23251.684.006.869.18301.684.016.859.14351.694.026.849.11常用缓冲溶液的配制方法1．甘氨酸–盐酸缓冲液（0.05mol/L）X毫升0.2mol/L甘氨酸+Y毫升0.2mol/LHCI，再加水稀释至200毫升pHXYpHXY2.02.42.62.850

2、50505044.032.424.216.83.03.23.43.65050505011.48.26.45.0甘氨酸分子量=75.07，0.2mol/L甘氨酸溶液含15.01克/升。 2．邻苯二甲酸–盐酸缓冲液（0.05mol/L）X毫升0.2mol/L邻苯二甲酸氢钾+0.2mol/LHCl，再加水稀释到20毫升pH(20℃)XYpH(20℃)XY2.22.42.62.83.0555554.0703.9603.2952.6422.0223.23.43.63.855551.4700.9900.5970.263邻苯二甲酸氢钾分子量=204.23，0.2m

3、ol/L邻苯二甲酸氢溶液含40.85克/升 3．磷酸氢二钠–柠檬酸缓冲液pH0.2mol/LNa2HPO4（毫升）0.1mol/L柠檬酸（毫升）pH0.2mol/LNa2HPO4（毫升）0.1mol/L柠檬酸（毫升）2.22.42.62.83.03.23.43.63.84.04.24.44.64.85.00.401.242.183.174.114.945.706.447.107.718.288.829.359.8610.3010.6018.7617.8216.8315.8915.0614.3013.5612.9012.2911.7211.1810.6

4、510.149.705.25.45.65.86.06.26.46.66.87.07.27.47.67.88.010.7211.1511.6012.0912.6313.2213.8514.5515.4516.4717.3918.1718.7319.1519.459.288.858.407.917.376.786.155.454.553.532.611.831.270.850.55Na2HPO4分子量=14.98，0.2mol/L溶液为28.40克/升。Na2HPO4-2H2O分子量=178.05，0.2mol/L溶液含35.01克/升。C4H2O7·H

5、2O分子量=210.14，0.1mol/L溶液为21.01克/升。4．柠檬酸–氢氧化钠-盐酸缓冲液pH钠离子浓度（mol/L）柠檬酸（克）C6H8O7·H2O氢氧化钠(克)NaOH97%盐酸（毫升）HCl（浓）最终体积（升）①2.23.13.34.35.35.86.50.200.200.200.200.350.450.3821021021021024528526684838383144186156160116106456810512610101010101010①    使用时可以每升中加入1克克酚，若最后pH值有变化，再用少量50% 氢氧化钠溶液或

6、浓盐酸调节，冰箱保存。②     5．柠檬酸–柠檬酸钠缓冲液（0.1mol/L）pH0.1  mol/L柠檬酸（毫升）0.1  mol/L柠檬酸钠（毫升）pH0.1  mol/L柠檬酸（毫升）0.1  mol/L柠檬酸钠（毫升）3.03.23.43.63.84.04.24.44.618.617.216.014.914.013.112.311.410.31.42.84.05.16.06.97.78.69.710.85.05.25.45.65.86.06.26.46.68.27.36.45.54.73.82.82.01.411.812.713.614.5

7、15.316.217.218.018.64.89.2柠檬酸C6H8O7·H2O：分子量210.14，0.1mol/L溶液为21.01克/升。柠檬酸钠Na3C6H5O7·2H2O：分子量294.12，0.1mol/L溶液为29.41克/毫升。6．乙酸–乙酸钠缓冲液（0.2mol/L）pH(18℃)0.2  mol/LNaAc(毫升)0.3  mol/LHAc(毫升)pH(18℃)0.2  mol/LNaAc(毫升)0.3  mol/LHAc(毫升)2.63.84.04.24.44.60.751.201.802.653.704.909.258.808.2

8、07.356.305.104.85.05.25.45.65.85.907.007.908.609.109.4