骨髓单个核细胞移植对脑梗死大鼠新血管形成的影响

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1、AbstractTheeffectoftransplantedbonemarrowmononuclearcellsonneoVasculogenesisinaratmodelofischemicstrokePostgraduate：LieYuSupervisor：JianpingWangDepartmentofNeurology,TheFifthClinicalCollegeZhengzhou，Hennan450052，ChinaBackgroundand0bjectiveBonemarrowmononuclearcells(BMMNCs)consistsofaheterogeneouspo

2、pulationofcelltypes．BMMNCswhichincludemultiplestemcellsandcandifferentiateintovesselwallcellsinthelimbischemiaandmyocardialinfarctioncanstronglypromoteneovasculogenesis．ThisstudyistoobservewhethertheBMMNCscanpromoteangiogenesisandarteriogenesisbydifferentiatingintoendothelialcellsandsmoothmusclecel

3、lsinaratmodelofischemicstrokeandwhetherBMMNCsexertlong—termneuroprotection．Materiaisandmethods1．Thetracer,isolation，identificationofcellularcomponentsandLabelingrateofBMMNCs．WeinjectedadultmaleSprague—DawleyratsintraperitoneallywithBrdU(50mg／kg／day)oncedailyfor14daysbeforeharvestingthebonemarrow；BM

4、MNCswereisolatedwithdensitygradientcentrifugationmethod；ThecharacterizationofCD34，CD45，CD90，CDl17andBrdUlabelingrateofBMMNCswereidentifiedwithflowcytometry．2．ExperimentalAnimalsandGroupsIVAdultmaleSprague-Dawleyrats(300-3109，3monthsold)wererandomlydividedintofourgroups：sham-operatedgroup(n=26)，untr

5、eatedischemicgroup(n=34)，vehicle—treatedischemicgroup(n=36)，andBMMNC-treatedischemicgroup(n=54)．3．Themodelofcerebralischemia，Celltransplantation，PreparationofsamplesandObservationindicatorThepMCAOmodelwasestablishedwithsutureembolimethod；BMMNCswereisolatedwithdensitygradientcentrifugationmethod．Bod

6、yweightchangeswereassessedweeklythroughday42afterstroke．TherecoveryofneurologicalfunctionaldeficitswereassessedasthesamedaysbymodifiedNeurologicalSeverityScores(mNSS)．Infarctvolumewasassessedonweek1with2％2，3，5一triphenyltetrazolium-chloride／saline叮C)staining．Thediameterandmorphologicalcharacteristic

7、sofleptomeningealanastomoses，thecircleofWillis，andbasilarartefieswerevisualizedbylatexperfusionondays14and42．Thedistribution，differentiation，arteriogeniceffectsofBMMNCswereidentifiedbyimmunofluorescencedoub