pgc-1α过表达在多巴胺能神经元变性中的保护作用

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1、分类号：R741.02学校代码：10392学科专业代:100204学号：210100231福建医科大学硕士研究生毕业论文PGC-1α过表达在多巴胺能神经元变性中的保护作用OverexpressionofPGC-1αintheprotectiveeffectofthedegenerationofdopaminergicneurons学位类型：医学硕士所在学院：协和临床医学院研究生：李东竹学科、专业：神经病学导师：叶钦勇副教授研究起止日期：2011年11月至2013年3月答辩日期：2013年05月31日二○一三年三月目录英文缩略词表········

2、···············································1中文摘要···························································3英文摘要··························································5引言·····························································81材料与方法······························

3、·····················102结果·······················································233讨论·······················································344结论·······················································37参考文献··························································38致谢······

4、······················································41综述····························································42英文缩略语表(Abbreviation)缩写英文全称中文全称AREaoxidantresponseelement抗氧化物应答元件DADopamine多巴胺GFPGreenfluorescentprotein绿色荧光蛋白MOImultiplicityofinfection感染系数+MPP1-methyl-4-p

5、henylpyridiniumion1-甲基-4-苯基-1,2,3,6-四氢吡啶离子MPTP1-Methyl-4-phenyl-1,2,3,6-tetrahydropyri-1-甲基-4-苯基-1,2,3,6-四氢吡啶dinemtDNAmitochondrialDNA线粒体DNAMTT3-(4,5)-dimethylthiahiazo3-(4,5(-z二甲基噻唑-y1)-3,5-di--2)-2,5-二苯基四phenytetrazoliumromide氮唑溴盐mt-TFAMitochondrialetranscriptiefactorA线粒体转

6、录因子ANRF1Nuclearrespiratoryfactor1核呼吸因子11PCRPolymerasechainreaction聚合酶链式反应PDParkinson’sDisease帕金森病PGC-1αPeroxisomeproliferator-activatedreceptor-coactivator过氧化物酶体增殖物激活受体-1辅激活因子1PMSFPhenylmethanesulfonylfluoride苯甲基磺酰氟ROSReactiveoxygenspecies活性氧THTyrosinehydroxylase酪氨酸羟化酶Tr

7、is2-amino-2-hydroxymethyl-1,3-propanediol三羟甲基氨基甲烷2PGC-1α过表达在多巴胺能神经元变性中的保护作用中文摘要【背景】帕金森病(Parkinson’sdisease,PD)是一种常见的中老年人神经系统退行性疾病。在帕金森病发病相关的众多环境与遗传因素中，线粒体功能障碍是一个共同点。因此，迅速控制线粒体功能的途径已成为潜在的治疗靶点。近年研究发现过氧化物酶体增殖物激活受体γ(PPARγ)辅激活因子1α(PGC-1α)在线粒体生物学过+程和氧化应激中具有突出的作用。PGC-1过表达通过调控线粒体功能

8、保护MPP诱导的SH-SY5Y细胞多巴胺能神经元变性未见报道。【目的】+PGC-1过表达对MPP诱导的SH-SY5Y细胞氧化损伤的保护作用及其分子机