抗赤霉病人工microRNA转基因小麦的创建

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1、分类号：密级：HUAZHONGAGRICULTURALUNIVERSITY硕士学位论文MASTER’SDEGREEDISSERTATION抗赤霉病人工microRNA转基因小麦的创建GENERATIONOFTRANSGENICWHEATWITHARTIFICIALMICRORNASAGAINSTFUSARIUMHEADBLIGHT研究生:张涛CANDIDATE：ZHANGTAO学号:2016301120010STUDENTNO.：专业:农业硕士MAJOR：MASTEROFAGRICULTURE导师:瞿波副教授SUPERVISOR：ASS

2、OCIATEPROFESSORQUBO廖玉才教授PROFESSORLIAOYUCAI中国武汉WUHAN，CHINA二○一八年六月JUNE，2018华中农业大学硕士学位论文抗赤霉病人工microRNA转基因小麦的创建GenerationofTransgenicWheatwithArtificialmicroRNAAgainstFusariumHeadBlight研究生:张涛学号:2016301120010指导教师:瞿波副教授廖玉才教授学位类型：农业硕士领域：作物华中农业大学植物科学技术学院中国·武汉CollegeofPlantScienc

3、es&TechnologyHuazhongAgruiculturalUniversityWuhan,China抗赤霉病人工microRNA转基因小麦的创建目录摘要................................................................................................................................iAbstract.....................................................

4、...................................................................iii缩略词表.......................................................................................................................v1前言..........................................................................

5、.................................................11.1小麦赤霉病的危害.........................................................................................11.2小麦抗赤霉病育种研究进展.........................................................................21.3小麦转基因技术研究进展.................

6、............................................................21.3.1基因枪介导法.......................................................................................31.3.2农杆菌介导法.......................................................................................41.4安全筛选体系...

7、..............................................................................................41.4.1安全筛选标记基因...............................................................................41.4.2最小表达框策略.......................................................................

8、............51.5RNA干扰技术及应用.............................................................................