矮牵牛dTph1转座子插入突变体库的扩容及突变体筛选

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1、HUAZHONGAGRICULTURALUNIVERSITY硕士学位论文MASTER’SDEGREEDISSERTATION矮牵牛dTph1转座子插入突变体库的扩容及突变体筛选AMPLIFICATIONOFdTph1TRANSPOSONINSERTIONMUTANTLIBRARYANDMUTANTSCREENINGOFPETUNIAHYBRIDA研究生:梁珊CANDIDATE：LIANGSHAN学号:2016305120006STUDENTNO.：专业:农业硕士MAJOR：MasterofAgriculture导师:刘国锋教授SUPERV

2、ISOR：PROF.LIUGUOFENG中国武汉WUHAN，CHINA二○一八年六月JUNE，2018分类号密级华中农业大学硕士学位论文矮牵牛dTph1转座子插入突变体库的扩容及突变体筛选AmplificationofdTph1TransposonInsertionMutantLibraryandMutantScreeningofPetuniahybrida研究生:梁珊学号:2016305120006指导教师:刘国锋教授学位类型:农业硕士领域：园艺华中农业大学园艺林学学院中国·武汉CollegeofHorticultureandFores

3、tryHuazhongAgriculturalUniversityWuhan,China矮牵牛dTph1转座子插入突变体库的扩容及突变体筛选目录摘要..........................................................................................................................................................iAbstract...................................

4、................................................................................................................iii缩略词表.................................................................................................................................................v第一章前言..

5、.........................................................................................................................................11.研究问题的由来.............................................................................................................................

6、...12.突变体创建的方法和途径................................................................................................................23.插入突变体库的创建........................................................................................................................43.1T-DNA插入

7、................................................................................................................................43.2转座子插入..............................................................................................................................44.Act1/d

8、Tph1转座系统研究进展....................................................................................