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1、M™ethodsinMolecularBiologySeriesEditorJohnM.WalkerSchoolofLifeSciencesUniversityofHertfordshireHatfield,Hertfordshire,AL109AB,UKForothertitlespublishedinthisseries,gotowww.springer.com/series/7651MicroRNAandCancerMethodsandProtocolsEditedbyWeiWuInstituteforBiocomplexityandInformatics,Departme
2、ntofBiologicalScience,UniversityofCalgary,Calgary,AB,CanadaEditorWeiWuInstituteforBiocomplexityandInformaticsDepartmentofBiologicalScienceUniversityofCalgaryCalgary,ABCanadawwu65@yahoo.com;wuwei@ucalgary.caISSN1064-3745e-ISSN1940-6029ISBN978-1-60761-862-1e-ISBN978-1-60761-863-8DOI10.1007/978-
3、1-60761-863-8SpringerNewYorkDordrechtHeidelbergLondonLibraryofCongressControlNumber:2010934277©SpringerScience+BusinessMedia,LLC2011Allrightsreserved.Thisworkmaynotbetranslatedorcopiedinwholeorinpartwithoutthewrittenpermissionofthepublisher(HumanaPress,c/oSpringerScience+BusinessMedia,LLC,233
4、SpringStreet,NewYork,NY10013,USA),exceptforbriefexcerptsinconnectionwithreviewsorscholarlyanalysis.Useinconnectionwithanyformofinformationstorageandretrieval,electronicadaptation,computersoftware,orbysimilarordissimilarmethodologynowknownorhereafterdevelopedisforbidden.Theuseinthispublication
5、oftradenames,trademarks,servicemarks,andsimilarterms,eveniftheyarenotidentifiedassuch,isnottobetakenasanexpressionofopinionastowhetherornottheyaresubjecttoproprietaryrights.Whiletheadviceandinformationinthisbookarebelievedtobetrueandaccurateatthedateofgoingtopress,neithertheauthorsnortheedito
6、rsnorthepublishercanacceptanylegalresponsibilityforanyerrorsoromissionsthatmaybemade.Thepublishermakesnowarranty,expressorimplied,withrespecttothematerialcontainedherein.Printedonacid-freepaperHumanaPressisapartofSpringerScience+BusinessMedia(www.springer.com)PrefaceThediscoveryofmicroRNAs(mi
7、RNAsormiRs)heraldedanewandanexcitingerainbiologyandstartedanewchapterinhumangeneregulation.ThemiRNAs,aclassofsmallendogenousnoncodingRNAs(~22nt),finetunethegeneexpressionattheposttran-scriptionallevelthroughmainlybinding3′-UTRofmRNAs.Theyarei
