谷氨酰胺对脓毒症小鼠氧化应激作用效果的研究

《谷氨酰胺对脓毒症小鼠氧化应激作用效果的研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、授予单位代码10089m学号或申请号丨3449HebeiMedicalUniversity硕士学位论文在职科学学位谷氨酰胺对脓毒症小鼠氧化应激作用效果的研究学位申请人：刘翠华导师：李增宁教授专业：营养与食品卫生学二级学院：第一医院2015年3月河北医科大学学位论文使用授权及知识产权归属承诺本学位论文在导师(或指导小组)的指导下，由本人独立完成。本学位论文研究所获得的研究成果，其知识产权归河北医科大学所有。河北医科大学有权对本学位论文进行交流、公幵和使用。凡发表与学位论文主要内容相关的论文,第一署名为单位河北医科大学，试验材料、原始数据、申报的专利等知识产权均归河北医科大学所有。否则，承担相

2、应法律责任。研究生签名^丨赞义导师签章：《名：级学院领导盖章：，丫年次月^^河北医科大学研究生学位论文独创性声明本论文是在导师指导下进行的研究工作及取得的研究成果,除了文中特别加以标注和致谢等内容外，文中不包含其他人已经发表或撰写的研究成果，指导教师对此进行了审定。本论文由本人独立撰写，文责自负。研究生签名：>樣7/导师签章：^0"年广月日目录中文摘要·············································································1英文摘要·······································

3、······································4英文缩写·············································································8研究论文谷氨酰胺对脓毒症小鼠氧化应激作用效果的研究前言·············································································9材料与方法···································································

4、·10结果·············································································16附图·············································································18附表·············································································23讨论··················································

5、···························25结论·············································································27参考文献·······································································27综述小鼠和人类脓毒症之间的差异·········································29致谢···················································

6、································44个人简历············································································45中文摘要谷氨酰胺对脓毒症小鼠氧化应激作用效果的研究摘要目的：探讨谷氨酰胺可否对脓毒症小鼠的氧化应激损伤起到减轻或者延缓，从而在危重症疾病氧化应激状态下发挥保护作用。方法：1动物分组与造模方法：96只昆明小鼠按完全随机方法分成4组，每组24只，分别设为空白组、模型组、谷氨酰胺低剂量组和谷氨酰胺高剂量组。空白组腹腔注射生理盐水，模型组和谷氨酰胺组腹腔注射内毒素制造脓

7、毒症动物模型，注射计量为5mg/kg；造型成功后模型组尾静脉注射生理盐水，谷氨酰胺低剂量组1h后尾静脉注射0.75g/kg谷氨酰胺，高剂量组1h后注射1.5g/kg谷氨酰胺。分别在1h、6h、12h眼眶取血后处死小鼠，解剖分离动物肝，用液氮迅速冷冻，之后放入-80℃低温冰箱保存。2谷胱甘肽过氧化物酶、超氧化酶歧化酶、丙二醛测定方法取4组小鼠血清、肝组织匀浆进行氧化应激指标超氧化酶歧化酶(superoxidedismuta