番鸭细小病毒与鹅细小病毒鉴别诊断方法的建立及初步应用

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1、2014୍36ज22௹ChinaPoultryVol.36đNo.22.2014ൌဒ࿹࣮◎◎ყٝ൵၄◎◎ثა׽Ѩཬ༥࿥ى༥ཬѨ׽࡫љᆐمٚ؎ࡹ৫ࠣԚႋႨ1đ231122*ٓ໓഻đ໱ဝषđٚ໓ఄđᇫূđޅᙛୡđଈૅধč1.ჹಸնള໾۽ӱႵཋ܄ඳđܼתჹ511517Ġ2.ܼ༆ն࿐׮໾॓࿐࠯ඌ࿐ჽđܼ༆ଲୡ530005Ġ3.תཔཅᎧഈూᆓ୪ࣜϷđࡾ༆ڢᇜ331801Ďᅋေğ۴ऌGenBank੣ى࿥༥ཬѨ׽čMDPVĎބث༥ཬѨ׽čGPVĎࠎၹቆ๝ჷ྽ਙ౵თࢲܒࠎၹREPၛࠣ٤๝ჷ྽ਙ౵თࢲܒࠎၹVP3đഡ࠹ਔ2ؓႄ໾đࡹ৫ਔ၂ᇕ࡫љᆐ؎MDPVބGPVᇗPCRٚمb

2、ھٚمؓGPVି๝ൈঔᄹԛ220bpބ443bpਆ่ோ؍đؓMDPVᆺିঔᄹԛ443bp၂่ோ؍đؓః෱ӈ࡮࿥ѨѨჰन໭หၳྟঔᄹbႋႨࡹ৫ٚمؓ36ٺਢԵѨਘࣉྛ࡟ҩđࢲݔ5ٺӯဝྟđఃᇏ1ٺູGPVဝྟđ4ݔࢲҩ࡟ਘѨაݔࢲ৖ٳ׽ѨđྟဝVPDMູٺ໖ކbၹՎđЧ൫ဒ෮ࡹ৫࡫љᆐ؎MDPVაGPVPCRٚمऎႵమᄝਢԵႋႨࡎᆴbܱ࡯Սğ؎ᆐљ࡫Ġႋّൔ৽ઽކऊĠ׽Ѩཬ༥ثĠ׽Ѩཬ༥࿥ىDevelopmentandPrimaryApplicationofDifferentialDiagnosticMethodforMuscovyDuckParvovirusa

3、ndGooseParvovirus1đ231122*FANWenshengđWUYangkaiđFANGWenqiđZHULeiđHEYiningđMOMeilanč1.QingyuanContainBioengineeringCo.đLtd.đQingyuanđGuangdong511517Ġ2.CollegeofAnimalScienceandTechnologyđGuangxiUniversityđNanningđGuangxi530005Ġ3.RuralEconomicManagementofficeofXushangqiaoTownđDongxian

4、gCountyđFuzhouđJiangxi331801ĎAbstractğToestablishamethodtodifferentiateMuscovyduckparvovirusčMDPVĎformgooseparvovirusčGPVĎđtheprimersREPU/DandVP3U/DweredesignedaccordingtothesequencesofREPandVP3genesavailableinGen⁃BankandaduplexPCRwasestablishedsuccessfullyfordetectionofMDPVandGPV.T

5、wobandsof443bpand220bpcouldbeamplifiedfromDNAsamplesofGPVđwhileonebandof443bpamplifiedfromDNAsamplesofMDPV.NobandcouldbeamplifiedfromduckhepatitisvirusđRiemerellaanatipestiferandduckplaguevirus.UsingtheduplexPCRđ5of36clinicalsampleswerepositiveand4wereMDPVandonewasGPVđwhichwasidenti

6、calwiththere⁃sultsofvirusisolation.TherefoređtheduplexPCRcouldbeusedtodifferentiateMDPVfromGPV.KeywordsğMuscovyduckparvovirusĠgooseparvovirusesĠPCRĠdifferentialdiagnosis൬۠ರ௹ğ2014-10-16ྩ߭ರ௹ğ2014-11-02∗๙࿟ቔᆀđE-mailğmomeilan@163.com-20-2014୍36ज22௹ChinaPoultryVol.36đNo.22.2014ൌဒ࿹࣮Ԡى࿥༥ཬѨ׽

7、Ѩđ෌ӫo೘ᇛѨpđ൞Ⴎى࿥1.3ႄ໾ഡ࠹აކӮ༥ཬѨ׽čMuscovyduckparvovirusđMDPVĎႄఏ۴ऌGenBankіMDPVބGPVಆࠎၹቆԠى࿥၂ᇕࠤྟࠇ࿰ࠤྟԮಙѨđၛڴ྅ބ྽ਙᇏЌ൯౵თREPࠎၹഡ࠹၂ؓ๙Ⴈႄ໾ŀ1ł԰గູᇶေหᆘbث༥ཬѨ׽čGooseparvovirusđREPU/DĠ۴ऌMDPVބGPVࠎၹቆ٤๝ჷ౵თGPVĎۋಙ൞၂ᇕᇶေ౓ݝཬثބԠى࿥ࠤྟࠇVP3ࠎၹഡ࠹၂ؓGPVหၳႄ໾VP3U/Dbႄ໾࿰ࠤྟaثအݝາభଢ൞đѨಙԮྟԨࢤۚြ྽ਙೂ༯ğREPUğ5′-ACCAATTCAATCAGGACGŀ2łᇶေԮಙѨ

8、ᆭ၂bᆃਆᇕၦѨन۳ඣౚအᆲြAA-3