骨髓间充质干细胞体外诱导培养后的电生理特性

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1、万方数据中国组织工程研究与临床康复多事73眷多亨27勇铲2009—07—02出版JoumalofClinicalRehabilitativeTissueEngineeringResearchJury2，2009VoL13,No．27j灾瓦震羲强匿掌骨髓间充质干细胞体外诱导培养后的电生理特性☆党懿，齐晓勇，孟存良，李树仁，李英肖，刘惠良，王天红Electrophysiologicalcharacteristicsofbonemaffowstemcellsfollowing／nv／troinductionDangY1，QiX

2、iao-yong．MengCun-liang，UShu怕n，UYing-xiao。UuHuHiang。Wang"rian-hongBACKGROUND：BonemarrowmesenchymaIstenlcells(BMSCs)cangrowinhostmyocardium，differentiateundermyocardialcondition，improvecardiacfunction．However，biologicaIcharacteristicsofBMSCdifferentiationarestilIun

3、dearpresently．OBJECTIVE：TOstudytheexpressionandelectrophysiologicalcharacteristicsofBMSCinvitroconnexin-43following5-azacitidine(5-aza)treatment．DESIGN。TIMEANDSETTING：111ecytologicalinvitrocontrolledstudywasperformedattheHeartCenter。HebeiProvindaIPeople’sHospitaI

4、的mJuly2007toFebruary2009．MATERIALS：Atotalof24malepigsaged2monthswerepurchasedfromExpecimentalAnimalCenter,Heb萌MedicaIUniversity．METHODS：BilateralfemoralbonemarrowwasobtainedfrOmpigsundersterilecondition．BMSCswereharvestedbyPercolIdensitygradientinv#ro．Atpassage2。

5、BMSCsweretreatedwith10pmol／L5．aza．andincubatedinDMeMwithoutinductor24hoursIater．1ndicesweremeasured1。2。3weeksfollowinginduction．A∞ntroIgroupwassetup，whichwasnottreatedwith5．azacitidine．Followingbonemarrowextraction．experimentalpigswereanesthetizedtoobtainventricu

6、larmusde．NormaIventricularmusdecellswerejsolatedandculturedbytissueblockenzymedigestionmathod．MAlN0UTCoMEMEASURES：Expressionofconnexin-43wasmeasuredbyImmunohistochomicaIstaining(ASCmethod)．Itocurrentdensityandactionpotentialweredaterminedbypatchdamptechnique．RESU

7、LTS：At1and2weeksfoIlowing5-azainduction．someBMSCswereposiUveforconnexin一43，withthepresenceofbrownpartidsssurroundingnudei．At3weeks．positiverateofconnexin-43was95％．TheareawithlargecelIdensitywaspresented枷thsimilarstructuretonorrnaImyocardium．At+80mV．comparedwithno

8、rmaImyocardialcells，ItocurrentdensitywassignificantlyreducedinBMSCsfollowing1and2weeksandinthecontrolgroup(P‘0．05)．Itocurrentdensitywassignificantlyincreasedto