内切酶活性表

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1、EnzymeSuppliedNEBuffer%ActivityinNEBuffers1234AatII405050100AccI4505010100Acc65I3+BSA107510025AciI3255010050AclI4+BSA10100100AfeI*SE-Y255025100AflII2+BSA5010025100AflIII3+BSA257510050AgeI1100501075AhdI4+BSA25750100AleI4102010100AluI210010075100AlwI45010010100AlwNI45010050100ApaI@

2、25°C4+BSA25500100ApaLI4+BSA10010010100ApoI@50°C3+BSA107510075AscI401010100AseI3NR75dd100NRAsiSI3+BSA5010010050AvaI4107510100AvaII4507510100AvrII210010050100BaeI@25°C2+BSA+SAM501005075BamHIUdd+BSA751005075BanI45010050100BanII41001050100BbsI21001002575BbvI21001002575BbvCI4501001010

3、0BceAIUdd+BSA25NRNR75BcgIU+SAM507510050BciVI41002510100BclI@50°C350100dd10075BfaI4755010100BfrBI4+BSA102510100BfuAI@50°C307510010BfuCI4+BSA1005025100BglI3507510050BglII3107510010BlpI45010010100Bme1580I2751007575BmgBI3+BSA02510010BmrI27510075100BmtI*SE-W251001050BpmI3+BSA751001001

4、00Bpu10I*SE-K02510025BpuEI@25°C2+SAM751001075BsaI@50°C37575100100BsaAI3757510075BsaBI@60°C275100100100BsaHI4+BSA50100100100BsaJI@60°C2100100100100BsaWI@60°C2+BSA501005050BsaXI47510010100BseRI210010075100BseYI3105010050BsgI4+SAM507550100BsiEI@60°C2+BSA5010010100BsiHKAI@65°C3+BSA50

5、100100100BsiWI@55°C310010010025BslI@55°C3105010075BsmI@65°C2751007575BsmAI@55°C35010010050BsmBI@55°C375100100100BsmFI@65°C4+BSA255050100BsoBI@65°C21010010050Bsp1286I4+BSA252525100BspCNI@25°C1+BSA+SAM100751075BspDI4257550100BspEI30101000BspHI41010050100BspMI3NRNR100NRBsrI@65°C3050

6、10010BsrBI250100100100BsrDI@60°C2+BSA501005075BsrFIU251007575BsrGI2+BSA2510010100BssHII@50°C3100100dd100100BssKI@60°C3+BSA05010050BssSI3050dd10010BstAPI*@60°CSE-W252510025BstBI@65°C4 755025100BstEII@60°C3507510075BstF5I*@65°CSE-Y755025100BstNI@60°C2+BSA1010010075BstUI@60°C2100100

7、50100BstXI@55°C32510010050BstYI@60°C25010075100BstZ17I3NRNR100NRBsu36I3+BSA0251000BtgI3+BSA2550100100BtsII4+BSA1005050100Cac8I3507510075ClaI4+BSA105050100DdeI37510010075DpnI410010075100DpnIIUNRNRdd100NRDraI4757550100DraIII3+BSA1007510025DrdI4255010100EaeI110010050100EagI310251001

8、0EarI110010050100EciI2+BSA5010010050EcoN