NEB内切酶产品具体Buffer活性表

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1、实用标准文案NEBufferActivityChartforRestrictionEnzymesNewEnglandBiolabsprovidesacolor-coded10XNEBufferwitheveryrestrictionendonucleasetoensure100%activity.Tohelpselectthebestconditionsfordoubledigests,thischartshowstheoptimal(supplied)NEBufferandapproximateactivityinthefourstandardNEBuffersforea

2、chenzyme.IfBSAissuppliedwithanenzyme,itisincludedinallNEBufferactivityreactions.Inaddition,thetablealsoshowsrecommendedreactiontemperature,heatinactivationtemperatureandrecommendeddiluentbuffer.Pleaseclickhereforadditionalinformationabout:UniqueNEBufferActivity  

3、DoubleDigestion

4、HeatInacti

5、vation

6、Activityat37°C

7、DiluentBuffers

8、Time-SaverEnzymes

9、HighFidelity(HF)RestrictionEnzymesThischarthasbeenupdatedtoreflectthechangeof54restrictionenzymestoNEBuffer4onNov.1,2008.ClickfortheChartLegend,IconDescriptionA

10、B

11、C

12、D

13、E

14、F

15、H

16、I

17、K

18、M

19、N

20、P

21、R

22、S

23、T

24、X

25、Z

26、EnzymeSuppliedNEBuffer%ActivityinNEBuffers

27、HeatInactiva-tion°CIncuba.Temp.°CDiluent1234AatIINEBuffer4050501006537AAcc65INEBuffer3+BSA1075100256537AAccINEBuffer45050101008037AAciINEBuffer32550100506537AAclINEBuffer4+BSA10100100No37BAcuINEBuffer4+SAM50100501006537BAfeINEBuffer42550251006537AAflIINEBuffer4+BSA50100251006537AAflIIINEBu

28、ffer3+BSA2575100508037BAgeINEBuffer11005010756537CAhdINEBuffer4+BSA257501006537AAleINEBuffer41025101006537AAluINEBuffer4100100751006537BAlwINEBuffer450100101006537AAlwNINEBuffer450100501006537AApaINEBuffer4+BSA255001006525AApaLINEBuffer4+BSA10010010100No37AApeKINEBuffer3257510050No75BApoIN

29、EBuffer3+BSA1075100758050A文档大全实用标准文案AscINEBuffer4010101006537AAseINEBuffer3NR75100ddNR6537BAsiSINEBuffer4+BSA501001001008037BAvaINEBuffer41075101008037AAvaIINEBuffer45075101006537AAvrIINEBuffer4100100501008037BEnzyme    topSuppliedNEBuffer%inB1%inB2%inB3%inB4HeatInactiva.Incuba.Temp.Diluen

30、tBaeGINEBuffer11007510506537ABaeINEBuffer4+BSA+SAM50100501006525ABamHINEBuffer3+BSA75100100dd100No37ABamHI-HF™NEBuffer41005010100No37ABanINEBuffer4+BSA50100501006537ABanIINEBuffer410010501008037ABbsINEBuffer210010025756537BBbvCINEBuffer450100101008037ABbvINEBu