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1、中国组织工程研究与临床康复第11卷第14期2007-04-08出版JournalofClinicalRehabilitativeTissueEngineeringResearchApril8,2007Vol.11,No.14CRTER基础医学脱细胞基质骨与成骨细胞和血管内皮细胞的生物相容性☆芮钢1,王者生2,尤元璋1,胡宝山1Biocompatibilityofacellularmatrixbonewithosteoblastandvascularendothelialcells1DepartmentofOrth
2、opedics,XiamenAbstractFirstHospital,FujianMedicalUniversity,AIM:Toobservethebiocompatibility,antigenicityandcytotoxicityofacellularmatrixporcineribsasthescaffoldmaterialXiamen361003,combinedwithrabbitosteoblastandvascularendothelialcells(VECs).FujianProvince,
3、China;2LiaoningMETHODS:TheexperimentwasconductedintheKeyLaboratoryforBiotherapyofHumanDiseasesofMinistryofProvincialPeople'sEducation,WestChinaHospitalofSichuanUniversityfromApriltoOctober2003.TheacellularmatrixporcineribswereHospital,Shenyangpreparedbyphysic
4、alandchemicalmethods.RabbitosteoblastandVECswereculturedinvitro.Therewerethree110016,LiaoningProvince,Chinagroupsinthisstudy.①Osteoblastgroup,whichcompoundedtheosteoblastwithacellularmatrixbone.②VECsgroup,whichcompoundedVECswithacellularmatrixbonematerial.③Os
5、teoblast+VECsgroup,whichcompoundedtheRuiGang☆,Doctor,Tutorofmaster,Chiefosteoblast+VECswithacellularbonematerial.④Controlgroup,whichwasthesimpleosteoblast.Ondays1,3,5and7,physician,Departmentthebiocompatibilityandantigenicityofacellularmatrixxenograftwereobse
6、rvedbyinvertedphasecontrastmicroscope,ofOrthopedics,XiamenFirstHospital,histologicalsectionsandscanningelectronmicroscope;theeffectsofthematerialsoncellcycleandDNAcontentwereFujianMedicaldetectedbyflowcytometrytoidentifyifthematerialshadtoxiceffectoncells.Uni
7、versity,Xiamen361003,FujianRESULTS:①ScanningelectronmicroscopeobservationofacellularmatrixbonesuggestedthatthetrabecularboneProvince,Chinastructureofacellularmatrixbonewascompletewithemptybonelacuna,andnocellcomponentswerefound.②Thereigang@yahoo.observationof
8、thegrowth,differentiationandproliferationofcellsunderinvertedphasecontrastmicroscopeappealedcom.cnthatwhenco-culturedwiththeacellularmatrixbonematerialfor12hours,thecellsofthreegroupsatta
