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1、BionanotechnologyII:fromBiomolecularAssemblytoApplications697FunctionalizednanoparticlesforbioanalysisbySERRSDuncanGraham*1,KarenFaulds*,DavidThompson*,FionaMcKenzie*,RobertStokes*,ColetteDalton*,RossStevenson*,JimAlexander†,PaulGarside†andEmmaMcFarlane†*Centr
2、eforMolecularNanometrology,DepartmentofPureandAppliedChemistry,WestCHEM,UniversityofStrathclyde,GlasgowG11XL,U.K.,and†StrathclydeInstituteofPharmacyandBiomedicalSciences,UniversityofStrathclyde,TheJohnArbuthnottBuilding,27TaylorStreet,GlasgowG40NR,U.K.Abstract
3、Metallicnanoparticlescanbeusedasbasicmaterialsforawidevarietyofpurposesincludingbuildingblocksfornanoassemblies,substratesforenhancedspectroscopiessuchasfluorescenceandRamanandaslabelsforbiomolecules.Inthepresentpaper,wereporthowsilverandgoldnanoparticlescanbef
4、unctionalizedwithspecificbiomolecularprobestointeractinaspecificmannerwithatargetmoleculetoprovideachangeinthepropertiesofthenanoparticleswhichcanbemeasuredtoindicatethemolecularrecognitionevent.ExamplesofthisapproachincludeDNAhybridizationtoswitchonSERRS(surfac
5、e-enhancedresonanceRamanscattering)whenaspecifictargetsequenceispresent,theuseofnanoparticlesforinvivoSERRSimagingandtheuseofnanoparticlesfunctionalizedwithantibodiestoprovideanewtypeofimmunoassay.Theseexamplesindicatehownanoparticlescanbeusedtoprovidehighlysen
6、sitiveandinformativedatafromavarietyofbiologicalsystemswhenusedincombinationwithSERRS.enhancementfactorofupto1010fortheRaman-scatteredIntroductionlight,andenhancementsofupto1014havebeenreportedSERRS(surface-enhancedresonanceRamanscattering)isanextremelysensiti
7、vedetectiontechnique,withsingle-molecule[2].detectionreported[1–3].SERRSisamoresensitivetechniqueThesensitivityofSERRSwouldbeofmassivebenefitinthanfluorescenceforthedetectionoflabelledDNAwhenthefieldsofmedicinebyfacilitatingtheearlydetectionofusingstandardlabo
8、ratory-basedequipment[4].Butthemaindiseasestatesanddirectdetectionofspecieswithincells.advantageoverfluorescenceisthatSERRSspectracontainHowever,toobtaineffectiveSERRS,ananalytecon
