ihc和fish检测胃癌her_2蛋白表达及基因扩增的比较_刘学政

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1、®世界华人消化杂志2011年9月8日;19(25):2679-2683wcjd@wjgnet.comISSN1009-3079(print)ISSN2219-2859(online)临床经验CLINICALPRACTICEIHC和FISH检测胃癌Her-2蛋白表达及基因扩增的比较刘学政,陈铭,张家均,董虹,柳文菊刘学政,陈铭,张家均,董虹,柳文菊,湖北省荆州市第一人expression(++),and29hadstrongexpression■背景资料民医院检验科湖北省荆州市434000胃癌在全世界肿(+++

2、).TherateofHer-2proteinoverexpression作者贡献分布:此课题由刘学政与陈铭设计;研究过程由刘学瘤死因中占第2was24.6%.Her-2geneamplificationwasdetect-政、张家均及董虹操作完成;研究所用试剂及分析工具由柳文位,大部分患者在菊提供;数据分析由刘学政完成;本论文写作由刘学政、陈铭及edin38cases,and80caseshadnoamplification.诊断时已属晚期,张家均完成.TherateofHer-2geneamplificatio

3、nwas32.2%.放化疗效果均不通讯作者:陈铭,434000,湖北省荆州市沙市区航空路8号,湖北理想.Her-2蛋白Of40casesofgastriccarcinomashowingno省荆州市第一人民医院检验科.chenmingbox@hotmail.com过表达和(或)基电话:0716-8067626Her-2proteinexpression,38(95%)hadnoHer-2因扩增不仅与胃收稿日期:2011-07-14修回日期:2011-09-06geneamplification(95%).Of3

4、3patientsshow-癌复发、转移和接受日期:2011-09-07在线出版日期:2011-09-08ingHer-2proteinmildexpression,30(90.9%)不良预后密切相hadnoHer-2geneamplification.Of16patients关,并且还是制定FluorescenceinsitushowingmoderateHer-2proteinexpression,9有效治疗方案如分子靶向治疗、hybridizationversushadnogeneamplification

5、.Of29patientsshow-激素治疗和化疗immunohistochemistryiningstrongHer-2proteinexpression,26(89.7%)的重要参考指标,thedetectionofHer-2genehadHer-2geneamplification.使Her-2表达的准确检测显得尤为amplificationandprotein重要.CONCLUSION:Thetotalcoincidenceratebe-expressioningastriccancertweenIHCa

6、ndFISHwas85.6%.IHCcanbeusedforinitialscreeningofHer-2expressionstatusinXue-ZhengLiu,MingChen,Jia-JunZhang,HongDong,Wen-JuLiugastriccancer.WhenmoderateorstrongHer-2proteinexpressionisdetected,itisnecessarytoconductFISHassaytoconfirmtheresult.Xue-ZhengLiu,Ming

7、Chen,Jia-JunZhang,HongDong,Wen-JuLiu,DepartmentofClinicalLaboratory,theFirstPeople’sHospitalofJingzhouCity,Jingzhou434000,KeyWords:Gastriccancer;Her-2;Immunohisto-HubeiProvince,Chinachemistry;FluorescenceinsituhybridizationCorrespondenceto:MingChen,Departme

8、ntofClinicalLaboratory,theFirstPeople’sHospitalofJingzhouCity,LiuXZ,ChenM,ZhangJJ,DongH,LiuWJ.FluorescenceNo.8HangkongRoad,ShashiDistrict,Jingzhou434000,insituhybridizationversusimmunohistochemistryint