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1、人胎阑尾淋巴组织及T、B淋巴细胞的发育李红，苏敏[基金项目]贵州省教育厅自然科学研究项目(黔教科2002116)[通信作者]苏敏，电话：（0851）6908567，E-mail：summ30@163.com，杨宇，胡蓉，黄悦，许庭良，姜俸蓉（550004贵阳，贵阳医学院组织学与胚胎学教研室）[摘要]目的探讨人胎阑尾内淋巴组织的发生以及T、B细胞的发育。方法收集人胎阑尾26例，HE染色观察微细结构，免疫组织化学SABC法和S-P法染色显示T、B细胞，用BioMiaspro图像分析软件进行计数。结果HE染色:9周胎儿阑尾管壁4层结构尚
2、未分化形成，上皮为复层柱状，间充质内有单个淋巴细胞散在分布；11周上皮内偶见单个淋巴细胞浸润，间充质内淋巴细胞较前略多；13～15周上皮内淋巴细胞浸润明显，初级淋巴小结雏形形成；16～20周管壁内初级淋巴小结及弥散淋巴组织明显；21～28周淋巴小结初级数量增多，尤以28周明显。免疫组化染色:9周间充质内出现少量IgM阳性细胞；11周出现CD3阳性细胞；15周IgM阳性反应细胞聚集开始形成淋巴小结，小结内CD3阳性反应细胞数量少；随胎龄增加，淋巴小结数量增多，IgM阳性反应细胞和CD3阳性反应细胞数量也逐渐数量增多（P＜0.01，P
3、＜0.05）。结论人胎阑尾淋巴组织发生的过程中，9周即有B细胞迁入，而T细胞在11周迁入。B细胞的发育早于T细胞。随胎龄增加，T、B细胞数量逐渐增多。[关键词]人胎；阑尾；B细胞；T细胞[中图法分类号][文献标志码]AHistogenesisofthelymphoidtissueanddevelopmentofTandBlymphocytesinhumanfetalappendixLiHong,SuMin,YangYu,HuRong,HuangYue,XuTingliang,JiangFengrong（DepartmentofHi
4、stologyandEmbryology,GuiyangMedicalUniversity,Guiyang,GuizhouProvince,550004,China）[Abstract]ObjectiveTostudyhistogenesisofthelymphoidtissueanddevelopmentofTandBlymphocytesinhumanfetalappendix.Methods26samplesofhumanfetalappendixfromthe9thto28thgestationalweekwerecoll
5、ected.Weobservedthefinestructurebyhematoxylinandeosinstaining.TandBlymphocyteswereidentifiedbyimmunohistochemistryusingthestreptavidin-peroxidaseorSABCmethod.Theimmunoreactivepositive-cellswerecountedusingBioMiasproimageanalysissoftware.Results①HE-staining：atgestation
6、alweek9,the4layersoftheappendixwerenotformed.Theepitheliumoftheappendixwasstratifiedcolumnarepithelium.Therewererareanddiffusedlymphocytesbetweenmesenchymalcells.Atweek11,thelymphocyteswereappearedintheepithelium.Theamountsoflymphocyteswereincreasingslightlyinthemesen
7、chyme.Thelymphocytesobviouslyinfiltratedintotheepitheliumfrom13to15week.Theprimarynoduleswereconsistedofagroupoflymphocytes.From16to20week,theprimarylymphoidnodulesanddiffuselymphoidtissuewereseenobviously.Atgestationalweek21to28,thenumberofprimarynodulesgraduallyincr
8、easedwithfetalage,reachingthepeakatweek28.②Immunohistochemistry:therewerefewIgM-positivecellswereobservedinthemesenchyme.Atg
