Proteomic analysis of exosomal cargo_ the challenge of high purity vesicle isolation

Proteomic analysis of exosomal cargo_ the challenge of high purity vesicle isolation

ID:40353622

大小:2.51 MB

页数:13页

时间:2019-07-31

Proteomic analysis of exosomal cargo_ the challenge of high purity vesicle isolation_第1页
Proteomic analysis of exosomal cargo_ the challenge of high purity vesicle isolation_第2页
Proteomic analysis of exosomal cargo_ the challenge of high purity vesicle isolation_第3页
Proteomic analysis of exosomal cargo_ the challenge of high purity vesicle isolation_第4页
Proteomic analysis of exosomal cargo_ the challenge of high purity vesicle isolation_第5页
资源描述:

《Proteomic analysis of exosomal cargo_ the challenge of high purity vesicle isolation》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库

1、MolecularBioSystemsViewArticleOnlineREVIEWViewJournalProteomicanalysisofexosomalcargo:thechallengeofhighpurityvesicleisolationCitethis:DOI:10.1039/c6mb00082gAgataAbramowicz,PiotrWidlakandMonikaPietrowska*There-discoveryofexosomesasintercellularmessengerswit

2、hhighpotentialfordiagnosticandtherapeuticutilityhasledtothembecomingapopulartopicofresearchinrecentyears.Oneoftheessentialresearchareasinthisfieldisthecharacterizationofexosomalcargo,whichincludesnumerousnon-randomlypackedproteinsandnucleicacids.Unexpectedl

3、y,averychallengingaspectofexplorationofextracellularvesicleshasturnedouttobetheireffectiveandselectiveisolation.Thepluralityofdevelopedprotocolsleadstoqualitativeandquantitativevariabilityintermsoftheobtainedexosomes,whichsignificantlyaffectstheresultsofdowns

4、treamanalysesandmakesthemdifficulttocompare,reproduceandinterpretbetweenresearchgroups.Currently,thereisageneralconsensusamongtheexosome-orientedcommunityconcerningtheurgentneedfortheoptimizationandstandardizationofmethodsemployedforthepurificationofthesevesi

5、cles.Hence,wereviewhereseveralstrategiesforexosomepreparationincludingultracentrifugation,chemicalprecipitation,affinitycapturingandfiltrationReceived1stFebruary2016,techniques.TheadvantagesanddisadvantagesofdifferentapproachesarediscussedwithspecialAccepted14

6、thMarch2016emphasisbeingplacedontheiradequacyforproteomicsapplications,whichareparticularlysensitivetoDOI:10.1039/c6mb00082gsamplequality.Weconcludethatcertainmethods,exemplifiedbyultracentrifugationcombinedwithiodixanoldensitygradientcentrifugationorgelfil

7、tration,althoughlabor-intensive,providesuperiorwww.rsc.org/molecularbiosystemsqualityexosomepreparationssuitableforreliableanalysisbymassspectrometry.Introductioninwardbuddingoflateendosomes/multivesicularbodiesandthataresecretedduringtheirfusionwiththeplas

8、maExosomes,verysmallvesicleswithgreatpotential,areoneofmembrane(reviewedindetailinref.2and3).However,afterthecomponentsoftheextracellularmilieuinvolvedininter-overthirtyyearsofintenseexosome-relatedres

当前文档最多预览五页,下载文档查看全文

此文档下载收益归作者所有

当前文档最多预览五页,下载文档查看全文
温馨提示:
1. 部分包含数学公式或PPT动画的文件,查看预览时可能会显示错乱或异常,文件下载后无此问题,请放心下载。
2. 本文档由用户上传,版权归属用户,天天文库负责整理代发布。如果您对本文档版权有争议请及时联系客服。
3. 下载前请仔细阅读文档内容,确认文档内容符合您的需求后进行下载,若出现内容与标题不符可向本站投诉处理。
4. 下载文档时可能由于网络波动等原因无法下载或下载错误,付费完成后未能成功下载的用户请联系客服处理。