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1、FoodandChemicalToxicologyxxx(2015)1e7ContentslistsavailableatScienceDirectFoodandChemicalToxicologyjournalhomepage:www.elsevier.com/locate/foodchemtoxFlowcytometryevaluationofinvitrocellularnecrosisandapoptosisinducedbysilvernanoparticlesabaa,*GirishKumar,HebaDeghe
2、idy,BrendanJ.Casey,PeterL.GoeringaOfficeofScienceandEngineeringLaboratories,CenterforDevicesandRadiologicalHealth,USFoodandDrugAdministration,SilverSpring,MD20993,USAbOfficeofCellular,Tissue,andGeneTherapies,CenterforBiologicEvaluationandResearch,USFoodandDrugAdminis
3、tration,SilverSpring,MD20993,USAarticleinfoabstractArticlehistory:Particlespossessuniquepropertiesinthenanoscale,e.g.,enhancedcatalyticactivity,highsurfacearea,Received28May2015andlightemission/absorptionproperties,thatmightresultininterferencewithcolorimetricinvit
4、roReceivedinrevisedformcytotoxicityassayssuchasMTT,XTTorMTS.Alternatively,assaysthatdonotusespectrophotometric15June2015detection,suchastrypanblueexclusionorflowcytometry(FC)basedassays,arelesslikelytobeAccepted17June2015influencedbynanoparticleinterference.Theaimoft
5、hisstudywastoevaluateFCassaystoassesstheAvailableonlinexxxcytotoxicityofthreedifferentsizes(10,100,or200nm)ofsilvernanoparticles(AgNPs)atdifferentmassconcentrations(1,25,or50ug/ml)inL-929fibroblastcells.After4hand24hexposure,cellnecrosisandKeywords:apoptosiswereasse
6、ssedusing7-AADandAnnexinVdyes,respectively,withFC.ThedataindicatethatFlowcytometryNanotoxicologycellnecrosisandapoptosisinAgNP-exposedfibroblastsdependsondose,exposuretime,andAgNPsize.SilvernanoparticlesThedataindicatethatAgNPsproducedadose-andtime-dependentdecrease
7、incellviability;however,Cytotoxicity10nmAgNPsweresignificantlymoretoxicthanlarger-sizedparticles.Thus,standardFCassayscanbeApoptosisutilizedtoassessapoptosisandnecrosisinresponsetonanomaterialexposure.PublishedbyElsevierLtd.1.Introductionthesafetyandefficacyofmedical
8、devicesincorporatingnanosilver.SafetyevaluationofmedicaldevicesisconductedpertheISOMetallicsilveratnanoscalehasveryuniquephysico-chemical10993-1s
