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1、病毒性胃肠炎患者中诺如病毒感染情况检cs测研究【摘要】目的：探讨病毒性胃肠炎患者中诺如病毒感染情况的检测分析。方法：通过对2013年1-9月于笔者所在医院进行治疗的120例病毒性胃肠炎患者，分别运用逆转录聚合酶链反应法检测和转录反转录协同实验检测，对两种检测方法的灵敏度、特异性进行比较。结果：运用转录反转录协同实验后GGI阳性7例，占5.83%,阴性113例，占94.17%,GGII阳性46例，占38.33%,阴性74例,占61.67%。转录反转录协同实验行检测，阳性53例，占44.17%,其中GGI患者7例，占阳性患者的13.21%,GGII患者46例，
2、占阳性患者的86.79%；恒温快速检测法检测出阳性患者53例,占总患者数的44.17%,其中不同性别、不同年龄段比较差异无统计学意义（P>0.05）,转录反转录协同实验的灵敏度及特异性分别为89.09%、93.85%,阳性预测值92.45%,阴性预测值91.04%o结论：临床运用转录反转录协同实验检测病毒性胃肠炎患者中诺如病毒感染情况，具有较高的灵敏度及特异性，临床值得推广。【关键词】病毒性胃肠炎;诺如病毒;感染；检测分析中图分类号R446文献标识码B文章编号1674-6805(2014)11-0053-03DetectionofViralGastroen
3、teritisPatientsNorVirusInfection/LIUYuan-xing,ZHENGZhi-yuan.//ChineseandForeignMedicalResearch,2014,12(11)：53-55[Abstract]Objective：Toinvestigatethedetectionandanalysisofviralgastroenteritispatientsnorvirusinfection.Method:120casesofviralgastroenteritispatientsfromJanuary2013toSep
4、tember2013inauthor?shospitalfortreatmentwereusingreversetranscriptionpolymerasechainreactionassayandreversetranscriptionusingthetranscriptioncollaborativeassayoftwospeciesdetectionsensitivity,specificitycompared.Result：TheuseofreversetranscriptioncollaborativeexperimentGGIpositive
5、patientswere7cases,accountingfor5.83%,negativepatientswere113cases,accountingfor94.17%,GGIIpositivepatientswere46cases,accountingfor38.33%,negativepatientswere74cases,accountingfor61.67%.Transcriptionreversetranscriptioncollaborativeexperimentallinedetection,positivepatientswere53
6、cases,accountingfor44.17%,whichincluded7casesGGIpatients,accountingfor13.21%,46casesGGIIpatients,accountingfor86.79%；thermostatrapidassaydetectedpositivethenumberofpatientswas53cases,accountingfor44.16%ofthetotalnumberofpatients,differentsexes,differentagesrelativelyhadnosignifica
7、ntdifference(P>0.05),reversetranscriptioncollaborativeexperimentalsensitivityandspecificitywere89.09%,93.85%,positivepredictivevaluewas92.45%,negativepredictivevaluewas91.04%.Conclusion:Theclinicaluseofreversetranscriptioncollaborativeassayinpatientswithneitherviralgastroenteritis
8、norvirusinfection,hashighsensitiv
