驳骨煎剂促进骨折愈合的实验研究

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1、驳骨煎剂促进骨折愈合的实验研究更新日期：2009-08-26点击：陈希　乔荣勤　曹亚飞提要　为探讨驳骨煎剂促进骨折愈合的作用机理。选用体重20～30g的NIH小白鼠120只，雌雄各半，随机分为正常组、给药组、对照组。除正常组外，其它各组均采用定点手法折骨术造成小鼠右下肢胫骨中段闭合性骨折。术后7、14、21、28天分批处死小鼠，处死前48小时，经腹腔注射同一批号0.2ml45Ca-CaCl2溶液，48小时后，断头取血，并解剖出左右胫骨，测定骨痂生长状况及钙沉积量的变化。结果显示，给药组与对照组，胫骨骨折处骨痂生长情况对比7天、14天时具有显著性意义(P＜0.05

2、)，到28天时，骨痂重量反而有所下降。表明驳骨煎剂具有促进骨折端的钙沉积，加速骨折愈合的作用。而认为这一作用是通过改善血液循环，促进血肿的吸收、机化，增加胶原的合成来实现的。主题词　骨折愈合/药物作用　驳骨煎剂/药效学　钙　实验研究　动物　小鼠中图分类号：R289.8；R683　文献标识码：A　文章编号：1001-6015(2000)-07-0005-02THESTIMULATIVEEFFECTOF“BOGUJIANJI”ONFRACTUREHEALING:AnExperimentalStudyChenXi，QiaoRongqin，CaoYafei（Orthop

3、edic-TraumatologicalHospitalAffiliatedtoGuangzhouTCMUniversity,Guangzhou510240）Inordertostudytheactionmechanismofthestimulativeeffectof“BOGUJIANJI”(BGJ,aTCDdecoctionforfracturehealing)onfracturehealing,120NIHwhitemice(MalesandFemales60each;BodyWeight:20-30grams)wererandomlydividedint

4、othreegroups:thenormal,theBGJ-treatedandthecontrol.ClosedfracturesoftherightmiddletibiaeweremadeintheBGJ-treatedandthecontrolgroupsbylocatedmanipulativeosteoclasty.Alltheanimalswerekilledinbatchesonthe7th,14th,21rstand28thdaysaftertheoperation.Forty-eighthoursbeforekilling,eachanimal

5、wasgiven0.2mlof45Ca-Cacl2solutionofthesamebatchthroughtransabdominalinjectionandkilled48hourslater.Thebloodwascollected,therightandlefttibiaeweredissected,andthechangesofthecallusgrowthandcalciumdepositweremeasured.Theresultsshowedthatcomparedwiththecontrolgroup,theweightofthecallusi

6、nthetibialfracturesiteoftheBGJ-treatedgroupwassignificantlyincreased(P<0.05)onthe7thand14thdayspostoperativelybutdecreasedonthe28thday.ItisconcludedthatBGJfunctionstostimulatethecalciumdepositionofthefragmentsandtospeedupthefracturehealing.ThisfunctionofBGJisaccomplishedthroughimprov

7、ingthebloodcirculation,promotingtheabsorptionandorganizationofthehematomaandincreasingthecollagensynthesis.KeyWords:　fracturehealing/drugaction　“BOGUJIANJI”/pharmacodynamics　calcium　experimentalstudy　animal　mouse　　驳骨煎剂是我院多年来用于治疗骨折、骨质疏松症合并骨折的经验方，经临床观察，疗效显著。骨折愈合是一个复杂的过程，而钙盐沉积是骨折愈合过程中必不

8、可少的重要步骤。为了进一