资源描述：
《乌司他丁对改善猪心肺复苏后炎症反应和心肺组织损伤的作用》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。
1、•论著•乌司他丁对改善猪心肺复苏后炎症反应和心肺组织损伤的作用武军元王烁李春盛袁伟【摘要】目的评价乌司他「(UT1)对猪心肺复苏(CPR)后炎症反应和心肺组织损伤的保护作用。方法雌性家猪20只•通过程控刺激法复制心室纤矗(家濒)动物模型.8min后开始CPR,fl主循环恢复(ROSC)的家猪按随机数字表法分为两组,UTI组复苏后即刻给予UTI100kU溶于生理盐水5nd,缓慢注射，3h1次，直到复苏后24h;对照组复苏后应用生理盐水5ml作为对照。分別于室颤前基础状态及HOSC后即刻、3、12.24h抽取家猪静脉血•采用
2、酶联免疫吸附试验(ELISA)测定肿斓坏死冈子-a(TNF-a)、门细胞介素-1p(IL-lP)、细胞间黏附分子・1(ICAM-1)和血管细胞黏附分子-KVCAM-1)的水平。ROSC后24h处死家猪，附取心、肺组织进行病理及超微结构观察。结果20只家猪经过Rmin空颤后复苏成功15只°UTI组8只和对照组7只存活24ho复苏后两组炎症因子均逐渐冋升.两组间室颤前和ROSC后即刻TNF-ot、IL-1BJCAM-1和VCAM-1比较差异无统计学意义;UT1组3h起TNF-a(ng/L)JL-1p(ng/L冰平明显低于对照
3、组(T、F-a：28.79±9.49比44.01±17.01,11,-1处153.50±67.47比252.09±80.411均Pv0.05),12h起ICAM-1(询,).VCAM-Kfxg/L)水平明显低于对照组(ICAM-1:11.05±5.11比17.09±5.69,VCAM-1：11.17±4.75比16.62±4.63,均Pv0.05)。ROSC后24h时IT1组心、肺组织损伤程度明显轻于对照组。结论复苏后应用UT1可以明显滅轻炎症反应和心肺组织损伤程度。【关键词】心肺复苏；乌司他丁；炎症反应；组织损伤；猪E
4、ffectofulinastatinonimprovinginflammatoryresponseandmyocardialandlungtissueinjuryaftercardiopulmonaryresuscitationinpigsWUJun-yuanWANGShuo.LIChun-sheng.YUANWei.DepartmentofEmergency9BeijingChaoyangHospital.CapitalMedicalUniversity,Beijing】00020.ChinaCorresponding
5、author：LIChun-sheng^Email：lcscy^yy@l63.com[Abstract]ObjectiveToevaluatetheeffectsofulinastatin(UTI)onimprovinginflammatoryresponseandmyocardialandlungtissueinjuryaftercardiopulmonaryresuscitation(CPR)inporcinemodel.MethodsVentricularfibrillation(VF)wasinducedin20
6、femalepigsbyprogrammedstimulationmethod・CPRwasbegun8minutesafterVF・Pigswithrecoveryofspontaneouscirculation(KOSC)wererandomlydividedintotwogroups:groupUTI.UTIwasgivenimmediatelyafterresuscitation,with100kUdissolvedin5mlofnormalsalinebyslowintravenousinjectionever
7、y3hours,upto24hoursafterresuscitation.Incontrolgroup,5mlofnormalsalinewasgivenwithsamedeliverytimeandfrequencyasthatintheUTIgroup.BeforeVF,immediatelyafterROSC.3hours,12hoursand24hoursafterROSC,samplesofvenousbloodwerecollectedforexaminationoftumornecrosisfactor-
8、a(TNF-a),interleukin—ip(IL-ip),intercellularadhesionmolecule-1(ICAM-1),andvascularcelladhesionmolecule-1(VCAM-1),usingtheenzyme-linkedimmunospecificassay(ELISA
