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1、高脂高糖与低剂量链月尿佐菌素诱导的糖尿病肾病大鼠模型的建［摘要］冃的建立高脂高糖?C低剂量链腺佐菌素(STZ)诱导的SD大鼠的早期糖尿病肾病(DN)模型，模拟早期DN的病理改变及肾功能改变。方法将SPF级正常雄性SD大鼠30只按照体重随机分为正常组、模型组。正常组大鼠给予普通饲料喂养，模型组大鼠高脂饲料喂养8周后，腹腔注射STZ35mg/kg,形成糖尿病模型后，继续高脂高糖饲料喂养4周后，观察两组大鼠肾脏病理改变、生化及肾功能各项指标的改变情况。结果模型组大鼠体重显著低于正常组(P<0.01),而随机血
2、糖值显著高于正常组(P<0.01),且达到糖尿病成模标准。模型组大鼠血清胰岛素水平显著低于正常组，相对肾重、24h尿微量白蛋白、Q1-微球蛋白、B2-巨球蛋白值显著高于正常组，差异有高度统计学意义(P<0.01)o模型组病理特征表现为肾小球基底膜增厚,系膜增宽，肾小球及肾小管糖原物质、胶原纤维聚集等。结论此造模方法可模拟早期DN的基本特征。本文采集自网络，本站发布的论文均是优质论文，供学习和研究使用，文中立场与本网站无关，版权和著作权归原作者所有，如有不愿意被转载的情况，请通知我们删除已转载的信息，如果
3、需要分享，请保晤本段说明。［关键词］高脂高糖；链腺佐菌素；糖尿病肾病［中图分类号］R587.2［文献标识码］A［文章编号］1673-7210(2017)06(b)-0012-04Establishmenlofhigh-fatandhigh-glucoseandlowdoseofStreptozocininducedratmodelsofdiabeticnephropathyYAOLanlZHANGYujie2,3WANGJian3LIUJing3LILinlin3WANGYe3LANYi4MAOXinm
4、in51.StaffRoomofChineseMedicinalResources,CollegeofTraditionalChineseMedicine,XinjiangMedicalUniversity,XinjiangUygurAutonomousRegion,Urumqi830011,China；2.DepartmentofPharmacy,theFirstAffiliatedHospitalofXinjiangMedicalUniversity,XinjiangUygurAutonomousR
5、egion,Urumqi830011,China；3.StaffRoomofPharmacology,CollegeofBasicMedicine,XinjiangMedicalUniversity,XinjiangUygurAutonomousRegion,Urumqi830011,China；4.TheSecondDepartmentofCadres,AffiliatedHospitalofTraditionalChineseMedicine,XinjiangMedicalUniversity,Xi
6、njiangUygurAutonomousRegion,Urumqi830000,China；5.CollegeofTraditionalChineseMedicine,XinjiangMedicalUniversity,XinjiangUygurAutonomousRegion,Urumqi830011,China［Abstract］ObjectiveToestablishtheearlydiabeticnephropathy(DN)ratmodelinducedbyhigh-fatandhigh-g
7、lucoseandlowdoseofStreptozocin(STZ),soastosimulatethepathologicandrenalfunctionchangesofearlyDN.MethodsThirtySPFlevelofnormalmaleSDratsweredividedintonormalgroupandmodelgroupbyweight・Normalgroupwasfedwithnormaldiet,mode1groupwasfedwithhigh-fatandhigh-glu
8、cosedietfor8weekscontinuously,then35mg/kgofSTZwasinjectedintraperitoneallytoestablishdiabeticratsmodel,afterthat,high-fatandhigh-glucosedietwasfedfor4weekscontinuously.Renalpathologicalchanges,biochemicalandrenalfunctionin
