bfgf对诱导大鼠心脏微血管内皮细胞血管的形成的影响

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1、trations(5，10，20，40ng／m1)，thenumberoftubeshigherthancontrolgroup，andinadose—dependentmanner(in0—20ng／m1)．Furthermore，aftertreat—mentwithbFGFinvaryingconcentrations(5，10，20，40ng／m1)for24h，theex—pressionofFit一1mRNAhigherthancontrolgroup，itparalleltothedevelopmentoftubestructu

2、rebyMatrigel．DiscussionTherapeuticangiogenesis，intheformofgrowthfactorproteinadministra—tionorgenetherapyhasemergedasanewmethodoftreatmentforpatientswithsevere，inoperableischenficheartdisease．Newvesselformationoccursthroughtheprocessesofangiogenesis，vasculogenesis，andarteri

3、ogenesis，underthecontrolofgrowthfactorssuchasthosethatbelongtotheVEGF，bFGF．bFGFasamemberoftheFGFfamily，isreleasedbymostcelltypes．Itin—ducesendothelialcellproliferation，migration，andformationofnewvessels．TheresearchresultsalsoshowthatCMECcanbeinducedtodeveloptubestruc—tm'eby

4、Matrigel．Additional，bFGFalsoimprovetheexpressionofVEGFanditsreceptors．VEGFfamilycurrentlycomprisessixmembers：VEGF—A、VEGF—B、VEGF—C、VEGF—D、VEGF—Eandplacentagrowthfactor(PIGF)．VEGFanditshigh—affinitybindingreceptors，thetyrosinekinaseFlk一1andFit一1，arethoughttobeimportantforthed

5、evelopmentofvasctrlature(endothelialcellpro—liferation，migration，basementmembranedegradationandformationofnewves—sels)．TherearelotsofreportsabouttheinfluenceofbFGFonVEGFR一2andfewreportsabouttheinfluenceofbFGFonVEGFR—1．TheFit一1signalingpathwaymaybeimportantincontrollingblood

6、vesselgrowthanddevelopment．TheresearchresultsalsoshowthatbFGFcaninhancetheexpressionofFh—lmRNA．ItiSacuet}latbFGFinfluenceneovascularl‘zationthroughVEGFR．Conclusion1．cardiacmicrovascularendothehalcellcanbeinducedtodeveloptube·5·structurebyMatrigelinvitro．2．bFGFcanpromotecard

7、iacmicrovascularendothelialcelltOformnewbloodvesselsandincertainrageshowingadose—dependentmanner3．bFGFcanenhancetheexpressionofFit——1andincertainrageshowingadose—dependentmanner，thisdose—dependentmannersimilartothatofnewbloodvessels7quantity．Keywordsbasicfibroblastgrowthfac

8、tor；vascularendothelialgrowthfactor；vascLllarendothelialgrowthfactorreceptor；cardi