gmcsf对诱导人血管内皮细胞血管形成的影响与vegf的作用

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1、understandingofhowASoriginandprogression．Currently，theroleofinflam-marioninAShasbeenacceptedwidely．Interactionsbetweenlipoprotein，manykindsofceⅡs，inflammatoryfactors，cytokines，growthfactorsgothoughthewholeprocess，finallyleadtounstable帅e，plaquedisruption．Andthefor-mationofun

2、stableplaqueistightlyconnectedwithnewlyformedbloodvesselswithintheplaque．Angiogenesisisaprocessbywhichpreexistingcapillariesproliferateandformnewcapillarynetworks，includingphysiologicalcondition(embryonicdevelopment)andpathologicalcondition(AS，tumorgrowth，woundhealing)．’rhi

3、8processincludematrixdegradation，endothelialceUsmi—grafion，proliferation，tubeformation．Themediatorofangiogenesiscanbeclas-siftedbytwokinds：angiogenicfactors，suchasVEGF，bFGF，ILandSOon；angiogenicinhibitor，suchasangiostatin，endostatinandSOon．ItwasbeforeO-veronehundredyearsthat

4、angiogenesiswithintheplaquehadbeendetected．Whenintimaexceedacertainthickness，newlyformedbloodvesselsoriginatefromadventifialvasavasorunlgoingthroughmedialayer．Withthedevelopmentoflesion，thenumber，volume，shapeofnewlyformedbloodvesselsalealsochanging．Theyprovidewaysforlipopro

5、teinandcontributetoplaquegrowth．Ⅵ‰atimportantisthatagoodmanyinflammatorycellsinfluxthroughthesenewlyformedvesselsintop】掣andproduceavarietiesofinflammatoryfactors，cyto-kines，growthfactors，andMMP8expressionup—regulation，whichaggressiveinflammatoryreactionswithintheplaque．More

6、over，thesenewlyformedbloodvesselslackSMCsandmuI翟leeHsthemselves，whichleadtoplaqueinstability，plaquedisruptionandaseriesofclinicalacutecomplications．GM—CSFasakindofintlammatoryfactorCanbesecretedbymacrophage，SMCs，ECswithintheplaque．强eexperimentculturedHUVECsinvitro，andsetupa

7、stableangiogenesisculturesystembyMatrigelinordertodiscussthein—fluencebroughtbyGM—CSFoninducinghumanvascularendothelialcellstoformnewbloodvesselsandtheroleofVEGF．ResultshadshowedthatwiththeincreaseofGM—CSFconcentrationsandtheaddingtime，thenumberoftubeswereonrise．Moreover，th

8、enumberoftubesalsoevidentlyincreasedafteraddinSVEGF．Researchesindicatedthattheeffe