bfgf对戊四氮诱导癫痫持续状态大鼠海马内神经发生及海马谷氨酸和γ-氨基丁酸含量的影响

《bfgf对戊四氮诱导癫痫持续状态大鼠海马内神经发生及海马谷氨酸和γ-氨基丁酸含量的影响》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、徐州医学院硕士擘住论文BrdU阳性细胞在NS组、NS+bFGF组主要分布于颗粒细胞层；在PTZ组、PTZ+bFGF两组，发作后7天，大部分BrdU免疫阳性细胞分布于齿状回颗粒细胞下层，处理后第14天，BrdU阳性细胞除了分布于颗粒细胞层以外，另有一部分位于门区和内分子层。新生细胞在由颗粒下层向颗粒层迁移的过程中，胞核变大，形状更接近圆形或卵圆形。阴性对照实验切片无特征性免疫反应产物。2、癫痫后海马谷氨酸、T．氨基丁酸含量变化：PTz组发作后7天、14天两日龄组海马组织谷氨酸的含量较NS组有显著增高(P

2、谷氨酸含量较PTz组下降妒o．05)。结论1．bFGF可使14日龄和60日龄大鼠SE后海马神经元发生增加，增生的细胞从颗粒下层大部分迁移到颗粒细胞层，还有一些迁移到门区和内分子层。2．bFGF能够降低14日龄和60日龄大鼠癫痫发作后异常增加的谷氨酸含量。关键词癫痫持续状态；碱性成纤维细胞生长因子；神经发生：海马；谷氨酸；t氨基丁酸4徐州医学院硕士学位论文Effectofba

3、sicfibroblastgrowthfactoronneurogenesisandthecontentofglutamicacidandT-aminobutyricacidinthehippocampusfollowingpent)rlenetetrazol—inducedstatusepilepticusofratsAbstractobjectiveTherenovationofvariouskindsofneurotrophicfactorandsterncellfactorafterseizuresareconcernedrecently．bFGF(basic

4、fibroblastgrowthfactor)asaneurotrophicfactor,Canreducecellularnecrosisandapoptosisafterbraininjury,sustainthesurvivalofneuronsroundtheinjuredregion．AndbFGFisconsideredtoplayneuroprotectiveeffectinbraininjury,buttheitsmechanismshavenotbeenfullyinvestigated．Thepuq)0seofthisarticleistostud

5、ytheeffectofbFGFonneurogenesisandthecontentofglutamicacidand1,-aminobutyricacidinthehippocampusfollowingpentylenetetrazol—inducedstatusepilepticusofdevelopingratS．MethodsPostnatalday(P)，P14andP60SpragueDawley(SD)ratswerereceivedPTZtomakestatusepilepticus(SE)，meanwhilenormalsaline蜘ection

6、够contrast．Theratswithvariousageweredividedrandomlyintomgroup、PTZ+bFGFgroup、NSgroupandNS+bFGFgroup．Weexcerptratsinabovegroupson7daysand14daysafterSE．WeusedBrdUtolabeledthenewlybomcellstodetecttheneurogenesisofdentategyrus．ThecontentofglutamicacidandT-aminobutiricacid(GABA)inhomogenizedbr

7、aintissueoftheratswasdeteminedwiththehighperformanceliquidchromatography．Results1．BrdUimmunohischemistry：QuantitativeanalysisofBrdUlabelingrevealedasignificantincreaseintheproliferationrateofneuralprecursorcellsinthedentategyruson7daysand14daysafterSE(P