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1、41.1.28A(d)Stoppers.—Syntheticrubberorcork.AOACOfficialMethod996.06(e)Mojonniercentrifugebasket.Fat(Total,Saturated,andUnsaturated)(f)Hengarmicroboilinggranules.inFoods(g)Baskets.—Aluminumandplastic.HydrolyticExtractionGasChromatographicMethod(h)Shakerwat
2、erbath.—Maintaining70–80°C.FirstAction1996(i)Steambath.—Supportingcommonglassware.Revised2001(j)Waterbath.—Withnitrogenstreamsupply,maintaining40±(Applicabletodeterminationoffatinfoods.)5°C.(k)Wristactionshaker.—DesignedforMojonniercentrifugeCaution:Boron
3、trifluoridemaybefatalifinhaled.baskets.(l)Mojonniermotordrivencentrifuge.—Optional;maintainingSeeTables996.06A–Cfortheresultsoftheinterlaboratorystudy600´g.supportingacceptanceofthemethod.(m)Gravityconvectionoven.—Maintaining100±2°C.A.Principle(n)Vortexmi
4、xer.Fatandfattyacidsareextractedfromfoodbyhydrolyticmethods(o)Gasdispersiontubes.—25mm,porosity“A,”extracoarse,(acidichydrolysisformostproducts,alkalinehydrolysisfordairy175mm.products,andcombinationforcheese).Pyrogallicacidisaddedto(p)Threedramvials.—Abo
5、ut11mL.minimizeoxidativedegradationoffattyacidsduringanalysis.(q)Phenolicclosedtopcaps.—Withpolyvinylliner,tofitvials.Triglyceride,triundecanoin(C11:0),isaddedasinternalstandard.Fat(r)Teflon/siliconesepta.—Tofitvials.isextractedintoether,thenmethylatedtof
6、attyacidmethylestersC.Reagents(FAMEs)usingBF3inmethanol.FAMEsarequantitativelymea-(a)Pyrogallicacid.suredbycapillarygaschromatography(GC)againstC11:0internal(b)Hydrochloricacid.—12Mand8.3M.Tomake8.3MHCl,standard.Totalfatiscalculatedassumofindividualfattya
7、cidsex-pressedastriglycerideequivalents.Saturatedandmonounsaturatedadd250mL12MHClto110mLH2O.Mixwell.Storeatroomtem-fatsarecalculatedassumofrespectivefattyacids.Monounsaturatedperature(20–25°C).fatincludesonlycisform.(c)Ammoniumhydroxide.—58%(w/w).(d)Dieth
8、ylether.—Purityappropriateforfatextraction.B.Apparatus(e)Petroleumether.—Anhydrous.(a)Gaschromatograph(GC).—Equippedwithhydrogenflame(f)Ethanol.—95%(v/v).ionizationdetector,capillarycolumn,splitmodeinjector,oventem-
