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1、45.4.12glomeratedwith350mofMicroBeadquaternaryAOACOfficialMethod2001.02amine-functionalizedlatex,orequivalent.Determinationoftrans-Galactooligosaccharides(c)pHmeter.(TGOS)inSelectedFoodProducts(d)Plasticvials.—50mL,withscrewcaps,resistanttotempera-Ion
2、-ExchangeChromatographyturesupto100C.FirstAction2001(e)Waterbaths.—Withshaker,maintaining602Cand80(Applicabletothequantitativedeterminationofadded2C.trans-galactooligosaccharidesinselectedfoodproducts.)(f)Helium.—Instrumentgrade,purity99.996%.SeeT
3、able2001.02Afortheresultsoftheinterlaboratorystudy(g)Syringes.—10mL,lowpressure,forusewith25mm,0.2msupportingtheacceptanceofthemethod.porositymembranefilters.(h)Pipets.—(1)20,100,and1000Lpipetwithdisposabletips.A.Principle(2)DisposablePasteurpipets,1
4、46mm.trans-Galactooligosaccharides(TGOS)andlactoseareextracted(i)Laboratorycentrifuge.—Toholdmicrotubes;operatingatfromatestportionwithhotphosphatebuffer.Theextractistreated11000g.with-galactosidasetohydrolyzeTGOSandlactose.Boththeini-(j)Benchcentrif
5、uge.—Tohold30mLtubes;operatingat1000g.tialandthetreatedsolutionareanalyzedusinghigh-performancean-(k)Dilutor.—Repeatedlydispensingsolutionsat1%accuracy.ion-exchangechromatographywithpulsedamperometricdetection(l)Microtubes.—Polypropylene1.5mLwithcap.
6、(HPAEC–PAD).Inthefirstassay,freegalactoseandlactosearede-(m)Testtubes.—Plastic,15mL,withscrewcaps.terminedintheinitialtestsolution.Inthesecondassay,thetotal(n)Ultrasoniccleaner.—Withdegascapability.amountofgalactosereleasedfromTGOSandlactoseisdetermine
7、d(o)Vortexmixer.inthetreatedsolution.TGOSarecalculatedfromconcentrationsofC.Reagentslactoseandgalactose.Fortestsolutionpreparation,extraction,andformobilephaseandre-B.ApparatusandMaterialsagentspreparation,use18MDIwaterthroughout.(a)High-performancean
8、ion-exchangechromatograph(a)Phosphatebuffer.—0.2M,pH6.0.Dissolve22.0gKH2PO4(HPAEC).—Liquidchromatographgradientpump,de-gasmodule,and6.0gK2HPO43H2Oinwateranddiluteto1L.Sterilize30minmicroinjectionvalve,pulsedelectrochemicaldetector(1.0m
