瓦里安系列7-瓦里安气相层析仪教材(繁体)

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1、氣相層析儀基礎課程講員:張世春E-mail:kyle.chang@tw.varian.com日期:1999年7月15日主辦:美商亞洲瓦里安科技(股)臺灣分公司課程表0930-1030:GC基本原理I1030-1050:休息1050-1200:GC基本原理II1200-1300:午餐時間1300-1410:軟體操作I1410-1420:休息1420-1530:軟體操作II1530-1550:休息1550-1630:Maintenance&Troubleshooting1630-1700:Q&AGasChromatography一.Pri

2、nciple&Theory二.Column三.Injector四.DetectorPrinciple&TheoryPrincipleDistributionofsamplecomponentsbetweenthemobilegasphaseandthestationaryphase.Somecomponentsinteractmorewiththestationaryphasecausingthemtomoveslowerthroughthecolumnandbecomeseparated.Separationofsamplecomp

3、onentsTheGCSystemCarrierGasInertHelium,argon,nitrogen,hydrogen.Choicedictatedbydetector,cost,availability.Pressureregulatedforconstantinletpressure.Flowcontrolledforconstantflowrate.InjectorSampleintroduction/vaporization.Syringeinjection—accurate,reproducible.Injection

4、techniquesaresampledependent.ColumnColumnOvenAccuratetemperaturecontrol(0.1ºC).Temperatureprogrammable.DetectorRespondstocomponents.Universalorselectivedetection.ChromatogramPlotofdetectorsignalversustime.ComponentsappearaspeaksfrombaselineAllowsmeasurementofpeakretent

5、iontimesandareas.Qualitativeanalysis-identityofcomponent.Quantitativeanalysis-amountofcomponent.GCTheoryResolutionDegreeofseparationbetweentwopeaks.Measureofpeakwidthanddistancebetweenpeaks.Capillarycolumnhasgreaterresolutionthanpackedcolumn.PeakShapeIdealpeakshapeisaGa

6、ussiancurve.StatisticaldistributionofMolecules.ColumnSelectivitySolubilityversusvolatility.Chemicalnatureofstationaryphase.Chemicalnatureofsamplecomponents.Vaporpressureofcomponents.tR2/tR11…Separationisachievable.ColumnSelectivityColumnEfficiencyMeasureofpeakbroade

7、ning.Quantitativelyexpressedasnumberofplates.Vapor-liquidequilibriumindistillation.Fortheabovebubbble-capcolumn,thenumberofplate3.PlatetheoryFactorsInfluencingColumnEfficiencyA.Eddydiffusion(multipatheffect).•Dependsonsize,shapeofpacking,andhowwellcolumnwaspacked.•Negl

8、igibleforcapillarycolumns.B.Longitudinaldiffusion.Moleculardiffusioningasphase.Stronglydependentonflowrate.C.R